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Literature summary for 3.2.1.26 extracted from
- Biochemical characterization of soluble acid and alkaline invertases from shoots of etiolated pea seedlings (2010), J. Integr. Plant Biol., 52, 536-548.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuSO4 | acid invertase is inhibited about 50% by 4.8 mM CuSO4. Alkaline invertase is inhibited by 0.6 mMCuSO4 up to 50% | Pisum sativum |  |
| HgCl2 | does not affect acid invertase activity, 0.05 mM HgCl2 inhibits alkaline invertase activity by about 50% | Pisum sativum | |
| Tris-HCl | Tris-HCl does not affect acid invertase activity up to 14 mM, 10 mM Tris-HCl inhibits alkaline invertase activity by about 50% | Pisum sativum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.8 | - |
sucrose | acid invertase, pH 6.5, 37°C | Pisum sativum | |
| 38.6 | - |
sucrose | alkaline invertase, pH 6.5, 37°C | Pisum sativum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Pisum sativum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
x * 22000 and x * 45000, acid invertase, x * 30000, alkaline invertase, SDS-PAGE | Pisum sativum |
| 30000 | - |
x * 22000 and x * 45000, acid invertase, x * 30000, alkaline invertase, SDS-PAGE | Pisum sativum |
| 45000 | - |
x * 22000 and x * 45000, acid invertase, x * 30000, alkaline invertase, SDS-PAGE | Pisum sativum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| both soluble acid and alkaline invertase | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| shoot | shoot of etiolated seedling | Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.82 | - |
pH 6.5, 37°C, alkaline invertase | Pisum sativum |
| 16.6 | - |
pH 6.5, 37°C, acid invertase | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| melizitose + H2O | acid invertase, 4.6% of the activity with sucrose, alkaline invertase, 3.1% of the activity with sucrose | Pisum sativum | ? | - |
? | |
| raffinose + H2O | acid invertase, 38.9% of the activity with sucrose, alkaline invertase, 40.0% of the activity with sucrose | Pisum sativum | beta-D-fructose + melibiose | - |
? | |
| sucrose + H2O | - |
Pisum sativum | beta-D-fructose + alpha-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 22000 and x * 45000, acid invertase, x * 30000, alkaline invertase, SDS-PAGE | Pisum sativum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
both acid and alkaline invertase | Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.3 | - |
acid invertase | Pisum sativum |
| 7.3 | - |
alkaline invertase | Pisum sativum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7.5 | acid invertase | Pisum sativum |
| 5 | 9 | alkaline invertase | Pisum sativum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pisum sativum | isoelectric focusing, alkaline invertase | - |
5.7 |
| Pisum sativum | isoelectric focusing, acid invertase | - |
7 |
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