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Literature summary for 3.2.1.26 extracted from

  • Martel, C.M.; Warrilow, A.G.; Jackson, C.J.; Mullins, J.G.; Togawa, R.C.; Parker, J.E.; Morris, M.S.; Donnison, I.S.; Kelly, D.E.; Kelly, S.L.
    Expression, purification and use of the soluble domain of Lactobacillus paracasei beta-fructosidase to optimise production of bioethanol from grass fructans (2010), Biores. Technol., 101, 4395-4402.
    View publication on PubMed
Search for more literature for 3.2.1.26

Cloned(Commentary)

Cloned (Comment) Organism
overexpression as truncated cytosolic protein in Escherichia coli Lacticaseibacillus paracasei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 levan pH 5.0, 37°C Lacticaseibacillus paracasei
15
-
 nystose pH 5.0, 37°C Lacticaseibacillus paracasei
15.6
-
 inulin pH 5.0, 37°C Lacticaseibacillus paracasei
49
-
 1-kestose pH 5.0, 37°C Lacticaseibacillus paracasei
392
-
 raffinose pH 5.0, 37°C Lacticaseibacillus paracasei
398
-
 sucrose pH 5.0, 37°C Lacticaseibacillus paracasei

Organism

Organism UniProt Comment Textmining
Lacticaseibacillus paracasei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant truncated cytosolic protein Lacticaseibacillus paracasei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-kestose + H2O
-
 Lacticaseibacillus paracasei D-fructose + ?
-
 ?
grass juice + H2O hydrolysis of polyfructose moieties in agriculturally-sourced grass juice with recombinant truncated cytosolic enzyme results in the release of more than 13 mg/ml more bioavailable fructose than is measured in untreated grass juice. Bioethanol yields from fermentation experiments with Brewer's yeast and grass juice plus enzymeaere more than 25% higher than those achieved using untreated grass juice feedstock Lacticaseibacillus paracasei ?
-
 ?
inulin + H2O
-
 Lacticaseibacillus paracasei D-fructose + ?
-
 ?
levan + H2O
-
 Lacticaseibacillus paracasei D-fructose + ?
-
 ?
additional information no substrate: stachyose, melizitose Lacticaseibacillus paracasei ?
-
 ?
nystose + H2O
-
 Lacticaseibacillus paracasei D-fructose + ?
-
 ?
raffinose + H2O
-
 Lacticaseibacillus paracasei D-fructose + melibiose
-
 ?
sucrose + H2O
-
 Lacticaseibacillus paracasei beta-D-fructose + alpha-D-glucose
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 50 recombinant truncated cytosolic protein Lacticaseibacillus paracasei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16
-
 raffinose pH 5.0, 37°C Lacticaseibacillus paracasei
64
-
 1-kestose pH 5.0, 37°C Lacticaseibacillus paracasei
84
-
 sucrose pH 5.0, 37°C Lacticaseibacillus paracasei
89
-
 levan pH 5.0, 37°C Lacticaseibacillus paracasei
95
-
 nystose pH 5.0, 37°C Lacticaseibacillus paracasei
106
-
 inulin pH 5.0, 37°C Lacticaseibacillus paracasei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 recombinant truncated cytosolic protein Lacticaseibacillus paracasei