Protein Variants | Comment | Organism |
---|---|---|
D22N | activity bleow detection limit with sucrose. Increase in transfructosylating activity | Saccharomyces cerevisiae |
F82W | 71% of wild-type activity | Saccharomyces cerevisiae |
N21S | 104% of wild-type activity with sucrose. Large increase in transfructosylating activity | Saccharomyces cerevisiae |
N228A | 138% of wild-type activity | Saccharomyces cerevisiae |
N228A/N21S | 20% of wild-type activity | Saccharomyces cerevisiae |
N228R | 23% of wild-type activity, no transfructosylating activity | Saccharomyces cerevisiae |
N228R/N21S | 4% of wild-type activity | Saccharomyces cerevisiae |
N24S | 17% of wild-type activity with sucrose. Large increase in transfructosylating activity | Saccharomyces cerevisiae |
P205V | 112% of wild-type activity with sucrose. Large increase in transfructosylating activity | Saccharomyces cerevisiae |
P205V/W19Y/N21S | 3% of wild-type activity with sucrose. Large increase in transfructosylating activity | Saccharomyces cerevisiae |
W19Y | 175% of wild-type activity with sucrose. Increase in transfructosylating activity | Saccharomyces cerevisiae |
W19Y/N21S | 41% of wild-type activity with sucrose. Large increase in transfructosylating activity | Saccharomyces cerevisiae |
W19Y/N21S/N24S | 2% of wild-type activity with sucrose. Large increase in transfructosylating activity | Saccharomyces cerevisiae |
W19Y/N24S | 8% of wild-type activity with sucrose. Large increase in transfructosylating activity | Saccharomyces cerevisiae |
W287Y | 4% of wild-type activity with sucrose. Increase in transfructosylating activity | Saccharomyces cerevisiae |
W291Y | 32% of wild-type activity with sucrose. Increase in transfructosylating activity | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19 | - |
sucrose | mutant N24S, pH 4.8, 50°C | Saccharomyces cerevisiae | |
38 | - |
sucrose | wild-type, pH 4.8, 50°C | Saccharomyces cerevisiae | |
1300 | - |
sucrose | mutant N21S, pH 4.8, 50°C | Saccharomyces cerevisiae | |
5000 | - |
sucrose | mutant W19Y/N21S, pH 4.8, 50°C | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | - |
Saccharomyces cerevisiae | beta-D-fructose + alpha-D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Suc2 | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
360 | - |
sucrose | mutant N24S, pH 4.8, 50°C | Saccharomyces cerevisiae | |
3300 | - |
sucrose | mutant W19Y/N21S, pH 4.8, 50°C | Saccharomyces cerevisiae | |
3430 | - |
sucrose | wild-type, pH 4.8, 50°C | Saccharomyces cerevisiae | |
4000 | - |
sucrose | mutant N21S, pH 4.8, 50°C | Saccharomyces cerevisiae |