Literature summary for 3.2.1.26 extracted from

Dipasquale, L.; Gambacorta, A.; Siciliano, R.A.; Mazzeo, M.F.; Lama, L.
Purification and biochemical characterization of a native invertase from the hydrogen-producing Thermotoga neapolitana (DSM 4359) (2009), Extremophiles, 13, 345-354.

Search for more literature for 3.2.1.26

Activating Compound

Activating Compound	Comment	Organism	Structure
dithiothreitol	1 mM, 134% of initial activity	Thermotoga neapolitana

Inhibitors

Inhibitors	Comment	Organism	Structure
Co2+	2 mM, 64% residual activity	Thermotoga neapolitana
Cu2+	2 mM, 5% residual activity	Thermotoga neapolitana
Fe3+	2 mM, 0.8% residual activity	Thermotoga neapolitana
guanidinium hydrochloride	2 M, 2% residual activity	Thermotoga neapolitana
Hg2+	2 mM, no residual activity	Thermotoga neapolitana
K+	2 mM, 79% residual activity	Thermotoga neapolitana
Urea	2 M, 29% residual activity	Thermotoga neapolitana
Zn2+	2 mM, 0.8% residual activity	Thermotoga neapolitana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
14	-	sucrose	85°C, pH 6.0	Thermotoga neapolitana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44298	-	1 * 47000, SDS-PAGE, 1 * 44298, MALDI TOF-MS	Thermotoga neapolitana
47000	-	gel filtration and PAGE	Thermotoga neapolitana

Organism

Organism	UniProt	Comment	Textmining
Thermotoga neapolitana	-	DSM 4359	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
51800	-	85°C, pH 6.0	Thermotoga neapolitana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
inulin + H2O	-	Thermotoga neapolitana	D-fructose + ?	-	?
raffinose + H2O	-	Thermotoga neapolitana	D-fructose + ?	-	?
sucrose + H2O	-	Thermotoga neapolitana	beta-D-fructose + alpha-D-glucose	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 47000, SDS-PAGE, 1 * 44298, MALDI TOF-MS	Thermotoga neapolitana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
85	-	-	Thermotoga neapolitana

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
80	85	retains at least 50% of initial activity for 6 h	Thermotoga neapolitana

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
7300000	-	sucrose	85°C, pH 6.0	Thermotoga neapolitana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Thermotoga neapolitana

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
520000	-	sucrose	85°C, pH 6.0	Thermotoga neapolitana

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Release 2023.1 (January 2023)