Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.26 extracted from

  • Gangadhara, A.R.; Ramesh Kumar, P.; Prakash, V.
    Influence of polyols on the stability and kinetic parameters of invertase from Candida utilis: correlation with the conformational stability and activity (2008), Protein J., 27, 440-449.
    View publication on PubMed
Search for more literature for 3.2.1.26

General Stability

General Stability Organism
polyol activated enzyme is stable up to 72 hours Cyberlindnera jadinii

Inhibitors

Inhibitors Comment Organism Structure
sorbitol inhibitory action at 30 and 40% solution Cyberlindnera jadinii
xylitol inhibitory action at 35 and 40% solution Cyberlindnera jadinii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3 3.5 sucrose native invertase, pre-incubation at 80°C for 20 min, 55°C, pH 4.5 Cyberlindnera jadinii
10.05
-
 sucrose 25% xylitol, 55°C, pH 4.5 Cyberlindnera jadinii
10.75
-
 sucrose 30% glycerol, 55°C, pH 4.5 Cyberlindnera jadinii
11.5
-
 sucrose 15% sorbitol, 55°C, pH 4.5 Cyberlindnera jadinii
12.5
-
 sucrose native invertase, 55°C, pH 4.5 Cyberlindnera jadinii
16.66
-
 sucrose 40% xylitol, pre-incubation at 80°C for 20 min, 55°C, pH 4.5 Cyberlindnera jadinii
17.85
-
 sucrose 40% glycerol, pre-incubation at 80°C for 20 min, 55°C, pH 4.5 Cyberlindnera jadinii
19.23
-
 sucrose 40% glycerol, 55°C, pH 9 Cyberlindnera jadinii
21.27
-
 sucrose 40% sorbitol, pre-incubation at 80°C for 20 min, 55°C, pH 4.5 Cyberlindnera jadinii
21.73
-
 sucrose 40% xylitol, 55°C, pH 9 Cyberlindnera jadinii
29.41
-
 sucrose native invertase, 55°C, pH 9 Cyberlindnera jadinii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + H2O Cyberlindnera jadinii
-
 D-fructose + D-glucose
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Glycerol no significant increase in alpha-helical content of invertase with 30% glycerol at optimal conditions, under pre-incubation at 80 degrees Celsius for 20 min 30% concentration increases alpha-helical content from 2-6% and tertiary structure is conserved under both conditions, increase of thermal denaturation temperature from 75 to 79, 82, 85, and 84 degrees Celsius with 10, 20, 30, and 40% glycerol, stability increase due to hydration Cyberlindnera jadinii
additional information with sorbitol no significant increase in alpha-helical content of invertase with 20% sorbitol at optimal conditions, under pre-incubation at 80 degrees Celsius for 20 min 30% concentration increases alpha-helical content from 2-5%, at pH 9 increases of alpha-helical content from 3-6% and tertiary structure is conserved under both conditions, increase of thermal denaturation temperature from 75 to 77, 79, 81, and 82 degrees Celsius with 10, 20, 30, 40% sorbitol, respectively, and from 70 to 72 and 73 degrees Celsius with 30 and 40% sorbitol, respectively, at pH 9, stability increase due to hydration Cyberlindnera jadinii
additional information xylitol with no significant increase in alpha-helical content of invertase with 25% xylitol at optimal conditions, under pre-incubation at 80 degrees Celsius for 20 min 30% concentration increases alpha-helical content from 2-6%, at pH 9 increases of alpha-helical content from 3-7% and tertiary structure is conserved under both conditions, increase of thermal denaturation temperature from 75 to 79, 82, and 84 degrees Celsius with 10, 20, and 30-40% xylitol and from 70 to 73, 75, 77 degrees Celsius with 20, 30, 40% xylitol at pH 9, stability increase due to hydration Cyberlindnera jadinii

Organism

Organism UniProt Comment Textmining
Cyberlindnera jadinii
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 maximum activity at 15% sorbitol (1.23-fold increase), increased activity from 5-20% solutions, 100 mM sucrose, 10 min, 55°C, 50 mM sodium acetate buffer, pH 4.5 Cyberlindnera jadinii
additional information
-
 maximum activity at 25% xylitol (1.57-fold increase), increased activity from 10-30% solutions, 100 mM sucrose, 10 min, 55°C, 50 mM sodium acetate buffer, pH 4.5 Cyberlindnera jadinii
additional information
-
 maximum activity at 30% glycerol (1.39-fold increase), increased activity from 10-40% solutions, 100 mM sucrose, 10 min, 55°C, 50 mM sodium acetate buffer, pH 4.5 Cyberlindnera jadinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + H2O
-
 Cyberlindnera jadinii D-fructose + D-glucose
-
 ?
sucrose + H2O 100 mM sucrose, 10 min, 55 degrees Celsius, 50 mM sodium acetate buffer, pH 4.5 Cyberlindnera jadinii D-fructose + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
invertase
-
 Cyberlindnera jadinii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 20 min, pH 9.0, thermal denaturation starts without solvent or with 20% sorbitol Cyberlindnera jadinii
72
-
 20 min, pH 9.0, thermal denaturation starts with 30% sorbitol Cyberlindnera jadinii
73
-
 20 min, pH 9.0, thermal denaturation starts with 20% xylitol or 40% sorbitol Cyberlindnera jadinii
75
-
 20 min, pH 4.5, thermal denaturation starts without solvent Cyberlindnera jadinii
75
-
 20 min, pH 9.0, thermal denaturation starts with 30% xylitol Cyberlindnera jadinii
77
-
 20 min, pH 4.5, thermal denaturation starts with 10% sorbitol Cyberlindnera jadinii
77
-
 20 min, pH 9.0, thermal denaturation starts with 40% xylitol Cyberlindnera jadinii
79
-
 20 min, pH 4.5, thermal denaturation starts with 10% glycerol or xylitol, or 20% sorbitol Cyberlindnera jadinii
80
-
 20 min, pH 4.5, 70% residual activity retained with 40% glycerol Cyberlindnera jadinii
80
-
 20 min, pH 4.5, 80% residual activity with 40% xylitol Cyberlindnera jadinii
80
-
 20 min, pH 4.5, maximum of 45% residual activity above 20% to 40% sorbitol Cyberlindnera jadinii
81
-
 20 min, pH 4.5, thermal denaturation starts with 30% sorbitol Cyberlindnera jadinii
82
-
 20 min, pH 4.5, thermal denaturation starts with 20% glycerol or xylitol, or 40% sorbitol Cyberlindnera jadinii
84
-
 20 min, pH 4.5, thermal denaturation starts with 30% and 40% xylitol or 40% glycerol Cyberlindnera jadinii
85
-
 20 min, pH 4.5, thermal denaturation starts with 30% glycerol Cyberlindnera jadinii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
93.3
-
 sucrose native invertase, 55°C, pH 9 Cyberlindnera jadinii
154
-
 sucrose native invertase, pre-incubation at 80°C for 20 min, 55°C, pH 4.5 Cyberlindnera jadinii
690.8
-
 sucrose 40% sorbitol, pre-incubation at 80°C for 20 min, 55°C, pH 4.5 Cyberlindnera jadinii
737.5
-
 sucrose 40% xylitol, 55°C, pH 9 Cyberlindnera jadinii
1055
-
 sucrose 40% glycerol, 55°C, pH 9 Cyberlindnera jadinii
1078
-
 sucrose 40% glycerol, pre-incubation at 80°C for 20 min, 55°C, pH 4.5 Cyberlindnera jadinii
1232
-
 sucrose 40% xylitol, pre-incubation at 80°C for 20 min, 55°C, pH 4.5 Cyberlindnera jadinii
1541
-
 sucrose native invertase, 55°C, pH 4.5 Cyberlindnera jadinii
1891
-
 sucrose 15% sorbitol, 55°C, pH 4.5 Cyberlindnera jadinii
2148
-
 sucrose 30% glycerol, 55°C, pH 4.5 Cyberlindnera jadinii
2428
-
 sucrose 25% xylitol, 55°C, pH 4.5 Cyberlindnera jadinii

pH Stability

pH Stability pH Stability Maximum Comment Organism
9
-
 94% activity loss of native enzyme Cyberlindnera jadinii
9
-
 no protecting effect of glycerol Cyberlindnera jadinii
9
-
 sorbitol retains 48% activity at 40% concentration Cyberlindnera jadinii
9
-
 xylitol retains 68% activity at 40% concentration Cyberlindnera jadinii