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Literature summary for 3.2.1.26 extracted from

  • Jia, L.; Zhang, B.; Mao, C.; Li, J.; Wu, Y.; Wu, P.; Wu, Z.
    OsCYT-INV1 for alkaline/neutral invertase is involved in root cell development and reproductivity in rice (Oryza sativa L.) (2008), Planta, 228, 51-59.
    View publication on PubMed
Search for more literature for 3.2.1.26

Cloned(Commentary)

Cloned (Comment) Organism
mutant gene amplification with phage P1-derived artificial chromosome clone P0451A10 for sequencing Oryza sativa

Protein Variants

Protein Variants Comment Organism
R205G mutant Oscyt-inv1 shows abnormal phenotypes with short primary, adventitious, and lateral roots (reduced cell lengths, disarray of cells but radial pattern conserved), floral transition delay, low seed setting rate, low pollen fertility Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63450
-
 calculated from DNA sequence Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + H2O Oryza sativa involved in root cell development and reproductivity in rice D-fructose + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
 japonica cv. Nipponbsare wild-type, F2 mutant from a cross of Oscyt-inv1 mutant with Oryza saliva induce cv. Kasalath wild-type
-

Purification (Commentary)

Purification (Comment) Organism
centrifugation after homogenization Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
kernel
-
 Oryza sativa
-
leaf
-
 Oryza sativa
-
root
-
 Oryza sativa
-
stem
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 mutant with 11.4% reduced neutral invertase activity compared to wild-type, with higher sucrose and lower glucose (-10% in leaves, -22% in roots) and fructose (-12% in leaves, -21% in roots) contents in 14-day-old mutant plants than in wild-type, part of the mutant phenotype is due to glucose deficit, mutant morphology phenotype is not fully expressed in 3% glucose medium Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + H2O involved in root cell development and reproductivity in rice Oryza sativa D-fructose + D-glucose
-
 ?
sucrose + H2O 1% sucrose, 50 mM potassium phosphate, pH 7, 37 degrees Celsius, 30 min Oryza sativa D-fructose + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
neutral invertase
-
 Oryza sativa

pI Value

Organism Comment pI Value Maximum pI Value
Oryza sativa calculated from DNA sequence
-
 6.79