General Stability | Organism |
---|---|
half-life in response to chymotrypsin attack: 315 min | Saccharum officinarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
160.6 | - |
sucrose | pH 5.0, 50°C, 55 mg/ml | Saccharum officinarum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
SDS-PAGE | Saccharum officinarum |
Organic Solvent | Comment | Organism |
---|---|---|
urea | 8 M urea at 20°C with a half-life of 74 min | Saccharum officinarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharum officinarum | - |
salt sensitive cultivar CP 77-400 | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, dialysis, Hiload anion exchange chromatography, elution at about 640 mM NaCl, hydrophobic interaction chromatography with phenyl superose column, and gel filtration chromatography on a Pharmacia FPLC unit | Saccharum officinarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | - |
Saccharum officinarum | D-fructose + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28000, native and subunit molecular mass almost identical on SDS-PAGE | Saccharum officinarum |
Synonyms | Comment | Organism |
---|---|---|
SAI | soluble acid invertase | Saccharum officinarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Saccharum officinarum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
53 | - |
half-life 112 min | Saccharum officinarum |
55 | - |
half-life 240 min | Saccharum officinarum |
63 | - |
half-life 10 min | Saccharum officinarum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21 | - |
sucrose | pH 5.0, 50°C | Saccharum officinarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2 | - |
- |
Saccharum officinarum |