Literature summary for 3.2.1.26 extracted from

Jegou, S.; Conreux, A.; Villaume, S.; Hovasse, A.; Schaeffer, C.; Cilindre, C.; Van Dorsselaer, A.; Jeandet, P.
One step purification of the grape vacuolar invertase (2009), Anal. Chim. Acta, 638, 75-78.

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Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
vacuole	-	Vitis vinifera	5773	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
71000	-	theoretical from nanoLC-chip-MS/MS analysis	Vitis vinifera

Organism

Organism	UniProt	Comment	Textmining
Vitis vinifera	-	Chardonnay, Champagne	-

Purification (Commentary)

Purification (Comment)	Organism
centrifugation of must and filtration through 0.45 micrometre membrane, dialysation against distilled water, ultrafiltration with hydrophilic polysulfone membrane with 10 kDa cutoff, concentrate freeze-dried, solubilized in 50 mM Tris-HCl buffer, pH 7.5, purified on Q-Sepharose Fast Flow anion exchange column, gradient of 0-500 mM NaCl in buffer, fractions of around 60 kDa pooled and desalted	Vitis vinifera

Source Tissue

Source Tissue	Comment	Organism	Textmining
fruit	-	Vitis vinifera	-

Synonyms

Synonyms	Comment	Organism
beta-D-fructofuranoside fructohydrolase	-	Vitis vinifera
vacuolar invertase 1	-	Vitis vinifera

pI Value

Organism	Comment	pI Value Maximum	pI Value
Vitis vinifera	calculated from sequence	-	4.6

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Release 2023.1 (January 2023)