Literature summary for 3.2.1.26 extracted from

Voegele, R.T.; Wirsel, S.; Moell, U.; Lechner, M.; Mendgen, K.
Cloning and characterization of a novel invertase from the obligate biotroph Uromyces fabae and analysis of expression patterns of host and pathogen invertases in the course of infection (2006), Mol. Plant Microbe Interact., 19, 625-634.

Search for more literature for 3.2.1.26

Activating Compound

Activating Compound	Comment	Organism	Structure
EDTA	slight activity increase	Uromyces viciae-fabae

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Saccharomyces cerevisiae and Pichia pastoris	Uromyces viciae-fabae

General Stability

General Stability	Organism
addition of up to 200 mM NaCl or 50 mM sucrose does not significantly alter INV1p stability	Uromyces viciae-fabae

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	virtually abolishes invertase activity	Uromyces viciae-fabae
additional information	Mg2+ does not affect the activity	Uromyces viciae-fabae
Na+	negatively effected by 0.8 M Na+	Uromyces viciae-fabae
Zn2+	slightly inhibitory	Uromyces viciae-fabae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
27.1	-	sucrose	in 100 mM Na-acetate, pH 4.6, and 100 mM sucrose, at 35°C	Uromyces viciae-fabae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
84300	-	estimated from amino acid sequence	Uromyces viciae-fabae

Organic Solvent Stability

Organic Solvent	Comment	Organism
Glycerol	addition of up to 20% glycerol does not significantly alter INV1p stability	Uromyces viciae-fabae

Organism

Organism	UniProt	Comment	Textmining
Uromyces viciae-fabae	Q2PCS4	precursor	-

Purification (Commentary)

Purification (Comment)	Organism
immobilized metal ion affinity chromatography	Uromyces viciae-fabae

Storage Stability

Storage Stability	Organism
-20°C, 4°C, or 20°C, in culture filtrate, several days, remains stable	Uromyces viciae-fabae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose + H2O	sucrose is the only substrate	Uromyces viciae-fabae	D-glucose + D-fructose	-	?

Synonyms

Synonyms	Comment	Organism
beta-D-fructofuranoside fructohydrolase	-	Uromyces viciae-fabae
INV1p	-	Uromyces viciae-fabae
invertase	-	Uromyces viciae-fabae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	slightly below 40°C	Uromyces viciae-fabae

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
20	-	temperatures above 20°C result in a rapid loss of enzyme activity	Uromyces viciae-fabae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.6	-	-	Uromyces viciae-fabae

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Release 2023.1 (January 2023)