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Literature summary for 3.2.1.26 extracted from

  • Lammens, W.; Le Roy, K.; Van Laere, A.; Rabijns, A.; Van den Ende, W.
    Crystal structures of Arabidopsis thaliana cell-wall invertase mutants in complex with sucrose (2008), J. Mol. Biol., 377, 378-385.
    View publication on PubMed
Search for more literature for 3.2.1.26

Cloned(Commentary)

Cloned (Comment) Organism
mutants are expressed in Pichia pastoris Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method using 22.5-28% PEG 8000 or 25% PEG 4000 as a precipitant in 0.1 M cacodylate pH 6.5 and 0.1 M zinc acetate Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
D239A shows a 6fold increase in KM and 1-kestose exohydrolase activity Arabidopsis thaliana
D23A inactive Arabidopsis thaliana
E203A inactive Arabidopsis thaliana
E203Q inactive Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
 Arabidopsis thaliana 5618
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
raffinose + H2O
-
 Arabidopsis thaliana D-fructose + melibiose
-
 ?
sucrose + H2O
-
 Arabidopsis thaliana D-glucose + D-fructose
-
 ?

Synonyms

Synonyms Comment Organism
cell-wall invertase 1
-
 Arabidopsis thaliana
cwINV1
-
 Arabidopsis thaliana