Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli and in an invertase-negative strain of Saccharomyces cerevisiae | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sucrose | increase of the sucrose concentration from 2.5 mM to 1 M results in decreased hydrolysis activity | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
sucrose | in 50 mM acetate buffer (pH 5.0) at 37°C | Aspergillus niger |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
SDS-PAGE | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | Q0ZR36 | putative intracellular invertase | - |
Purification (Comment) | Organism |
---|---|
His tag affinity chromatography | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-kestose + H2O | - |
Aspergillus niger | D-fructose + ? | - |
? | |
additional information | does not use inulin and levan as substrates | Aspergillus niger | ? | - |
? | |
nystose + H2O | - |
Aspergillus niger | D-fructose + ? | - |
? | |
sucrose + H2O | - |
Aspergillus niger | D-glucose + D-fructose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
invertase | - |
Aspergillus niger |
SucB | shows also transfructosylation activity of 20 to 30% of total activity at sucrose concentrations up to 400 mM | Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 40 | - |
Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | at 50°C or higher, no activity can be detected, whereas at lower temperatures, the SucB specific activity remains relatively high, with 50% activity remaining at 25°C | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Aspergillus niger |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
268.1 | - |
sucrose | in 50 mM acetate buffer (pH 5.0) at 37°C | Aspergillus niger |