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Literature summary for 3.2.1.26 extracted from
- Purification and some properties of beta-fructofuranosidase from Aspergillus niger IMI 303386 (2005), Process Biochem., 40, 2461-2466.
No PubMed abstract available
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Aspergillus niger IMI 303386 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 17% carbohydrate content | Aspergillus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from strain IMI 303386 49.8fold to homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 51.67 | - |
purified enzyme | Aspergillus niger |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 120000-130000, two enzyme forms, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aspergillus niger |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
temperature profile | Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified enzyme, pH 5.5, over 90% remaining activity after 5 h, rapid inactivation above | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Aspergillus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH profile | Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | purified enzyme, 40°C, 90% remaining activity after 5 h, rapid loss of activity below pH 4.0 and above pH 8.0 | Aspergillus niger |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus niger | enzyme form 1, isoelectric focusing | - |
4.4 |
| Aspergillus niger | enzyme form 1, isoelectric focusing | - |
5.4 |
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