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Literature summary for 3.2.1.26 extracted from
- Purification, biochemical and immunological characterization of acid invertases from apple fruit (2005), J. Integr. Plant Biol., 47, 50-59.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| isozymes SAI and CWI, DNA and amino acid sequence determinations and analysis | Malus domestica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, isozymes SAI and CWI, isozyme Sai has a lower KM for sucrose and a higher KM for raffinose compared to isozyme CWI | Malus domestica |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | cell wall-bound ivertase isozyme, CWI | Malus domestica | 5618 | - |
| soluble | soluble acid invertase isozyme, SAI | Malus domestica | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
4 * 50000, isozymes SAI and CWI, SDS-PAGE | Malus domestica |
| 220000 | - |
isozymes SAI and CWI | Malus domestica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| raffinose + H2O | Malus domestica | - |
D-fructose + melibiose | - |
ir |  |
| sucrose + H2O | Malus domestica | - |
D-glucose + D-fructose | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Malus domestica | - |
two isozymes from cell wall and soluble fraction | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native isozymes soluble acid invertase SAI and cell wall-bound invertase CWI from fruits to homogeneity | Malus domestica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Malus domestica | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrate specificity of isozymes SAI and CWI, no activity with stachyose of isozymes SAI and CWI | Malus domestica | ? | - |
? | |
| raffinose + H2O | - |
Malus domestica | D-fructose + melibiose | - |
ir | |
| sucrose + H2O | - |
Malus domestica | D-glucose + D-fructose | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 50000, isozymes SAI and CWI, SDS-PAGE | Malus domestica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cell wall-bound ivertase | - |
Malus domestica |
| CWI | - |
Malus domestica |
| invertase | - |
Malus domestica |
| SAI | - |
Malus domestica |
| soluble acid invertase | - |
Malus domestica |
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