Literature summary for 3.2.1.26 extracted from

Alberto, F.; Bignon, C.; Sulzenbacher, G.; Henrissat, B.; Czjzek, M.
The three-dimensional structure of invertase (beta-fructosidase) from Thermotoga maritima reveals a bimodular arrangement and an evolutionary relationship between retaining and inverting glycosidases (2004), J. Biol. Chem., 279, 18903-18910.

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination, expression of His-tagged wild-type and selenomethionine-labeled enzyme in Escherichia coli strain BL21(DE3)	Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged wild-type and selenomethionine-labeled enzyme, vapour diffusion method, 11 mg/ml wild-type enzyme in 15% PEG 1000, 150 mM Li2SO4, and 100 mM sodium citrate, pH 4.2, 3 days, 20°C, conditions for the selenomethionine-labeled enzyme: 8 mg/ml protein in 17% PEG 1000, 50 mM Li2SO4, 1% isopropanol, and 100 mM sodium citrate, pH 4.2, X-ray diffraction structure determination and analysis at 2.0-2.2 A resolution	Thermotoga maritima

Crystallization (Comment)

Organism

purified recombinant His-tagged wild-type and selenomethionine-labeled enzyme, vapour diffusion method, 11 mg/ml wild-type enzyme in 15% PEG 1000, 150 mM Li2SO4, and 100 mM sodium citrate, pH 4.2, 3 days, 20°C, conditions for the selenomethionine-labeled enzyme: 8 mg/ml protein in 17% PEG 1000, 50 mM Li2SO4, 1% isopropanol, and 100 mM sodium citrate, pH 4.2, X-ray diffraction structure determination and analysis at 2.0-2.2 A resolution

Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sucrose + H2O	Thermotoga maritima	-	D-glucose + D-fructose	-	?
sucrose + H2O	Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589	-	D-glucose + D-fructose	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	O33833	-	-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589	O33833	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged wild-type and selenomethionine-labeled enzyme from Escherichia coli strain BL21(DE3)	Thermotoga maritima

Reaction

Reaction	Comment	Organism	Reaction ID
raffinose + H2O = beta-D-fructofuranose + 6-O alpha-D-galactopyranosyl-alpha-D-glucopyranose	the enzyme reaction mechanism with retention of the anomeric configuration at the site of cleavage, active site structure	Thermotoga maritima	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose + H2O	-	Thermotoga maritima	D-glucose + D-fructose	-	?
sucrose + H2O	-	Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589	D-glucose + D-fructose	-	?

Subunits

Subunits	Comment	Organism
More	three-dimensional structure analysis	Thermotoga maritima

Synonyms

Synonyms	Comment	Organism
beta-fructosidase	-	Thermotoga maritima
invertase	-	Thermotoga maritima
More	the enzyme belongs to the glycoside hydrolase family GH32	Thermotoga maritima