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Literature summary for 3.2.1.26 extracted from

  • Liu, C.; Huang, L.; Chang, C.; Sung, H.
    Purification and characterization of soluble invertases from suspension-cultured bamboo (Bambusa edulis) cells (2006), Food Chem., 96, 621-631.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin activates isozyme IT II best at 0.2 mg/ml, and isozyme IT I slightly at 100 mg/ml Phyllostachys edulis
concanavalin A activates isozyme IT II best at 0.9 mg/ml Phyllostachys edulis
DTNB 0.5 mM, 11% activation of isozyme IT II, 24% inhibition of isozyme IT I Phyllostachys edulis
urease activates isozyme IT II best at 0.1 mg/ml Phyllostachys edulis

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 0.5 mM, 62% inhibition of isozyme IT I, 86% inhibition of isozyme IT II Phyllostachys edulis
Ag+ 1 mM, 56% inhibition of isozyme IT I, 81% inhibition of isozyme IT II Phyllostachys edulis
aniline 5 mM, 36% inhibition of isozyme IT I, 17% inhibition of isozyme IT II Phyllostachys edulis
Ca2+ 5 mM, 35% inhibition of isozyme IT I, 84% inhibition of isozyme IT II Phyllostachys edulis
Cu2+ 1 mM, 53% inhibition of isozyme IT I, 85% inhibition of isozyme IT II Phyllostachys edulis
D-fructose product inhibition, 2.5 mM, 62% inhibition of isozyme IT I, 70% inhibition of isozyme IT II Phyllostachys edulis
D-glucose product inhibition, 2.5 mM, 58% inhibition of isozyme IT I, 72% inhibition of isozyme IT II Phyllostachys edulis
DTNB 0.5 mM, 24% inhibition of isozyme IT I, 11% activation of isozyme IT II Phyllostachys edulis
Hg2+ 1 mM, 93% inhibition of isozyme IT I, 95% inhibition of isozyme IT II Phyllostachys edulis
iodoacetamide 5 mM, about 20% inhibition of isozyme IT I, 44% inhibition of isozyme IT II Phyllostachys edulis
Mg2+ 1 mM, 11% inhibition of isozyme IT I, 14% inhibition of isozyme IT II Phyllostachys edulis
PMSF 0.5 mM, 20% inhibition of isozyme IT I, 60% inhibition of isozyme IT II Phyllostachys edulis
pyridoxine 5 mM, 48% inhibition of isozyme IT I, 75% inhibition of isozyme IT II Phyllostachys edulis
sucrose substrate inhibition, 400 mM: 10% inhibition of isozyme IT I, 20% inhibition of isozyme IT II, 1 M: 10% inhibition of isozyme IT I, 62% inhibition of isozyme IT II Phyllostachys edulis
Zn2+ 1 mM, no inhibition of isozyme IT I, 86% inhibition of isozyme IT II Phyllostachys edulis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7
-
sucrose pH 5.0, 37°C, isozyme IT II Phyllostachys edulis
10.9
-
sucrose pH 7.0, 37°C, isozyme IT I Phyllostachys edulis

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble soluble alkaline and acid invertase isozymes, i.e. IT I and IT II Phyllostachys edulis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
4 * 60000, isozyme IT I, SDS-PAGE Phyllostachys edulis
67000
-
1 * 67000, isozyme IT II, SDS-PAGE Phyllostachys edulis
68000
-
isozyme IT II, gel filtration Phyllostachys edulis
240000
-
isozyme IT I, gel filtration Phyllostachys edulis

Organism

Organism UniProt Comment Textmining
Phyllostachys edulis
-
isozymes alkaline and acid invertases, i.e. IT I and IT II
-

Purification (Commentary)

Purification (Comment) Organism
native soluble alkaline isozyme IT I 144fold from cell suspension culture to homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration, and native soluble acid invertase isozyme IT II 130fold from cell suspension culture to homogeneity by ammonium sulfate fractionation, anion exchange and cation exchange chromatography, ConA affinity chromatography, and anion exchange chromatography Phyllostachys edulis

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Phyllostachys edulis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26
-
purified isozyme IT II Phyllostachys edulis
28.8
-
purified isozyme IT I Phyllostachys edulis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information both isozymes IT I and IT II show no activity with maltose Phyllostachys edulis ?
-
?
raffinose + H2O isozymes IT I and IT II Phyllostachys edulis alpha-D-galactosyl-1,6-alpha-D-glucose + D-fructose
-
?
sucrose + H2O isozymes IT I and IT II Phyllostachys edulis D-glucose + D-fructose
-
?

Subunits

Subunits Comment Organism
monomer 1 * 67000, isozyme IT II, SDS-PAGE Phyllostachys edulis
tetramer 4 * 60000, isozyme IT I, SDS-PAGE Phyllostachys edulis

Synonyms

Synonyms Comment Organism
acid invertase
-
Phyllostachys edulis
alkaline invertase
-
Phyllostachys edulis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 40 both isozyme IT I and isozyme IT II Phyllostachys edulis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
10 min, both isozymes IT I and IT II retain over 70% activity Phyllostachys edulis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
acid invertase isozyme IT II Phyllostachys edulis
7 7.5 alkaline invertase isozyme IT I Phyllostachys edulis

pI Value

Organism Comment pI Value Maximum pI Value
Phyllostachys edulis alkaline invertase isozyme IT I, isoelectric focusing
-
4.8
Phyllostachys edulis acid invertase isozyme IT II, isoelectric focusing
-
7.4