Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | activates isozyme IT II best at 0.2 mg/ml, and isozyme IT I slightly at 100 mg/ml | Phyllostachys edulis | |
concanavalin A | activates isozyme IT II best at 0.9 mg/ml | Phyllostachys edulis | |
DTNB | 0.5 mM, 11% activation of isozyme IT II, 24% inhibition of isozyme IT I | Phyllostachys edulis | |
urease | activates isozyme IT II best at 0.1 mg/ml | Phyllostachys edulis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 0.5 mM, 62% inhibition of isozyme IT I, 86% inhibition of isozyme IT II | Phyllostachys edulis | |
Ag+ | 1 mM, 56% inhibition of isozyme IT I, 81% inhibition of isozyme IT II | Phyllostachys edulis | |
aniline | 5 mM, 36% inhibition of isozyme IT I, 17% inhibition of isozyme IT II | Phyllostachys edulis | |
Ca2+ | 5 mM, 35% inhibition of isozyme IT I, 84% inhibition of isozyme IT II | Phyllostachys edulis | |
Cu2+ | 1 mM, 53% inhibition of isozyme IT I, 85% inhibition of isozyme IT II | Phyllostachys edulis | |
D-fructose | product inhibition, 2.5 mM, 62% inhibition of isozyme IT I, 70% inhibition of isozyme IT II | Phyllostachys edulis | |
D-glucose | product inhibition, 2.5 mM, 58% inhibition of isozyme IT I, 72% inhibition of isozyme IT II | Phyllostachys edulis | |
DTNB | 0.5 mM, 24% inhibition of isozyme IT I, 11% activation of isozyme IT II | Phyllostachys edulis | |
Hg2+ | 1 mM, 93% inhibition of isozyme IT I, 95% inhibition of isozyme IT II | Phyllostachys edulis | |
iodoacetamide | 5 mM, about 20% inhibition of isozyme IT I, 44% inhibition of isozyme IT II | Phyllostachys edulis | |
Mg2+ | 1 mM, 11% inhibition of isozyme IT I, 14% inhibition of isozyme IT II | Phyllostachys edulis | |
PMSF | 0.5 mM, 20% inhibition of isozyme IT I, 60% inhibition of isozyme IT II | Phyllostachys edulis | |
pyridoxine | 5 mM, 48% inhibition of isozyme IT I, 75% inhibition of isozyme IT II | Phyllostachys edulis | |
sucrose | substrate inhibition, 400 mM: 10% inhibition of isozyme IT I, 20% inhibition of isozyme IT II, 1 M: 10% inhibition of isozyme IT I, 62% inhibition of isozyme IT II | Phyllostachys edulis | |
Zn2+ | 1 mM, no inhibition of isozyme IT I, 86% inhibition of isozyme IT II | Phyllostachys edulis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | - |
sucrose | pH 5.0, 37°C, isozyme IT II | Phyllostachys edulis | |
10.9 | - |
sucrose | pH 7.0, 37°C, isozyme IT I | Phyllostachys edulis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | soluble alkaline and acid invertase isozymes, i.e. IT I and IT II | Phyllostachys edulis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
4 * 60000, isozyme IT I, SDS-PAGE | Phyllostachys edulis |
67000 | - |
1 * 67000, isozyme IT II, SDS-PAGE | Phyllostachys edulis |
68000 | - |
isozyme IT II, gel filtration | Phyllostachys edulis |
240000 | - |
isozyme IT I, gel filtration | Phyllostachys edulis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phyllostachys edulis | - |
isozymes alkaline and acid invertases, i.e. IT I and IT II | - |
Purification (Comment) | Organism |
---|---|
native soluble alkaline isozyme IT I 144fold from cell suspension culture to homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration, and native soluble acid invertase isozyme IT II 130fold from cell suspension culture to homogeneity by ammonium sulfate fractionation, anion exchange and cation exchange chromatography, ConA affinity chromatography, and anion exchange chromatography | Phyllostachys edulis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Phyllostachys edulis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26 | - |
purified isozyme IT II | Phyllostachys edulis |
28.8 | - |
purified isozyme IT I | Phyllostachys edulis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | both isozymes IT I and IT II show no activity with maltose | Phyllostachys edulis | ? | - |
? | |
raffinose + H2O | isozymes IT I and IT II | Phyllostachys edulis | alpha-D-galactosyl-1,6-alpha-D-glucose + D-fructose | - |
? | |
sucrose + H2O | isozymes IT I and IT II | Phyllostachys edulis | D-glucose + D-fructose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 67000, isozyme IT II, SDS-PAGE | Phyllostachys edulis |
tetramer | 4 * 60000, isozyme IT I, SDS-PAGE | Phyllostachys edulis |
Synonyms | Comment | Organism |
---|---|---|
acid invertase | - |
Phyllostachys edulis |
alkaline invertase | - |
Phyllostachys edulis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | both isozyme IT I and isozyme IT II | Phyllostachys edulis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10 min, both isozymes IT I and IT II retain over 70% activity | Phyllostachys edulis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
acid invertase isozyme IT II | Phyllostachys edulis |
7 | 7.5 | alkaline invertase isozyme IT I | Phyllostachys edulis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Phyllostachys edulis | alkaline invertase isozyme IT I, isoelectric focusing | - |
4.8 |
Phyllostachys edulis | acid invertase isozyme IT II, isoelectric focusing | - |
7.4 |