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Literature summary for 3.2.1.26 extracted from
- The novel non-glycosylated invertase from Candida utilis (the properties and the conditions of production and purification) (2002), Biochim. Biophys. Acta, 1594, 40-53.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.54 | - |
sucrose | pH 4.7, 30°C, F-form, extracellular | Cyberlindnera jadinii |  |
| 2 | - |
sucrose | pH 4.7, 30°C, S-form, extracellular | Cyberlindnera jadinii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Cyberlindnera jadinii | - |
- |
| intracellular | - |
Cyberlindnera jadinii | 5622 | - |
| periplasmic space | - |
Cyberlindnera jadinii | 42597 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
F-form, non-denaturing PAGE | Cyberlindnera jadinii |
| 62500 | - |
1 * 62500, F-form, SDS-PAGE | Cyberlindnera jadinii |
| 102000 | - |
2 * 102000, S-form, SDS-PAGE | Cyberlindnera jadinii |
| 280000 | - |
S-form, nondenaturing PAGE | Cyberlindnera jadinii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyberlindnera jadinii | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | S-form is a glycoprotein | Cyberlindnera jadinii |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| F-form and S-form | Cyberlindnera jadinii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | invertase F-form and invertase S-form | Cyberlindnera jadinii | - |
| culture medium | - |
Cyberlindnera jadinii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1497 | - |
S-form | Cyberlindnera jadinii |
| 2560 | - |
F-form | Cyberlindnera jadinii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no hydrolysis of lactose and maltose | Cyberlindnera jadinii | ? | - |
? | |
| sucrose + H2O | - |
Cyberlindnera jadinii | beta-D-fructose + alpha-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 102000, S-form, SDS-PAGE | Cyberlindnera jadinii |
| monomer | 1 * 62500, F-form, SDS-PAGE | Cyberlindnera jadinii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
S-form and F-form of invertase | Cyberlindnera jadinii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 80 | 30°C: about 80% of maximal activity of F-form of invertase, about 75% of maximal activity of S-form of invertase, 80°C: about 55% of maximal activity of F-form of invertase, 98% of maximal activity of S-form of invertase | Cyberlindnera jadinii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.4 | - |
S-form and F-form of invertase | Cyberlindnera jadinii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | pH 3.0: about 40% of maximal activity of F-form of invertase, about 50% of maximal activity of S-form of invertase, pH 6.0. about 40% of maximal activity of F-form of invertase, about 75% of maximal activity of S-form of invertase | Cyberlindnera jadinii |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Cyberlindnera jadinii | isoelectrofocusing, S-form and F-form | 4.8 | 4.7 |
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