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Literature summary for 3.2.1.26 extracted from

  • Zarate, V.; Belda, F.
    Characterization of the heterologous invertase produced by Schizosaccharomyces cerevisiae (1996), J. Appl. Bacteriol., 80, 45-52.
    View publication on PubMed
Search for more literature for 3.2.1.26

Cloned(Commentary)

Cloned (Comment) Organism
expression in Schizosaccharomyces pombe Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall in Schizosaccharomyces pombe the heterologous enzyme is secreted into the cell wall Saccharomyces cerevisiae 5618
-

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Schizosaccharomyces pombe
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Schizosaccharomyces pombe
glycoprotein the heterologous enzyme contains 65% carbohydrate, presence of galactose in a ratio of about 1:1 galactose:mannose Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + H2O
-
 Saccharomyces cerevisiae D-fructose + D-glucose
-
 ?
sucrose + H2O
-
 Schizosaccharomyces pombe D-fructose + D-glucose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 heterologous enzyme Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 stable below Saccharomyces cerevisiae
50
-
 heterologous enzyme is stable below Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Saccharomyces cerevisiae
4
-
-
 Schizosaccharomyces pombe
4.5
-
 heterologous invertase Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 7 30°C, 90 min, heterologous enzyme, stable Saccharomyces cerevisiae