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Literature summary for 3.2.1.26 extracted from
- Purification and properties of an extracellular invertase from Azotobacter chroococcum (1991), Enzyme Microb. Technol., 13, 267-271.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aniline | - |
Azotobacter chroococcum |  |
| Ba2+ | - |
Azotobacter chroococcum | |
| Ca2+ | - |
Azotobacter chroococcum | |
| Cd2+ | - |
Azotobacter chroococcum | |
| Cu2+ | - |
Azotobacter chroococcum | |
| Ni2+ | - |
Azotobacter chroococcum | |
| pyridoxal 5'-phosphate | - |
Azotobacter chroococcum | |
| pyridoxamine | - |
Azotobacter chroococcum | |
| Zn2+ | - |
Azotobacter chroococcum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Azotobacter chroococcum | - |
- |
| intracellular | - |
Azotobacter vinelandii | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
gel filtration | Azotobacter chroococcum |
| 59000 | - |
1 * 59000, SDS-PAGE | Azotobacter chroococcum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Azotobacter chroococcum | - |
ATCC 4412 | - |
| Azotobacter vinelandii | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | enzyme can be freed of its carbohydrate component without loss of its catalytic activity | Azotobacter chroococcum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Azotobacter chroococcum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.02 | - |
- |
Azotobacter chroococcum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + H2O | - |
Azotobacter vinelandii | D-fructose + D-glucose | - |
? | |
| sucrose + H2O | - |
Azotobacter chroococcum | D-fructose + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 59000, SDS-PAGE | Azotobacter chroococcum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Azotobacter chroococcum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 100 | - |
sucrose | - |
Azotobacter chroococcum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Azotobacter chroococcum |
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Release 2023.1 (January 2023)
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