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Literature summary for 3.2.1.26 extracted from

  • West, C.; Wade, M.; McMillan III, C.; Albersheim, P.
    Purification and properties of invertases extractable from Phytophthora megasperma var. sojae mycelia (1980), Arch. Biochem. Biophys., 201, 25-35.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 62% inhibition at 0.01 mM, 89% inhibition at 1 mM Phytophthora megasperma
Hg2+ 44% inhibition at 0.01 mM, 96% inhibition at 1 mM Phytophthora megasperma
PCMB 0.1 mM, significant inhibition Phytophthora megasperma

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8
-
sucrose
-
Phytophthora megasperma

Metals/Ions

Metals/Ions Comment Organism Structure
Cu(NO3)2 0.001-1 mM stimulates Phytophthora megasperma

Organism

Organism UniProt Comment Textmining
Phytophthora megasperma
-
var. sojae
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein glycoprotein with a protein to carbohydrate ratio of 3.2. Presence of mannose and glucose as the major components along with smaller amounts of galactose Phytophthora megasperma

Purification (Commentary)

Purification (Comment) Organism
-
Phytophthora megasperma

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Phytophthora megasperma
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
117
-
-
Phytophthora megasperma

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
raffinose + H2O
-
Phytophthora megasperma fructose + melibiose
-
?
sucrose + H2O
-
Phytophthora megasperma D-fructose + D-glucose
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
Phytophthora megasperma

pH Range

pH Minimum pH Maximum Comment Organism
4.2 7.4 50% of maximal activity at pH 4.2 and at pH 7.4 Phytophthora megasperma