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Literature summary for 3.2.1.26 extracted from
- Purification and properties of invertase from a glutamate-producing bacterium (1986), J. Ferment. Technol., 64, 285-291.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | activates | Corynebacterium glutamicum |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | CuCl2 | Corynebacterium glutamicum | |
| FeSO4 | - |
Corynebacterium glutamicum | |
| Hg2+ | HgCl2 | Corynebacterium glutamicum | |
| Ni2+ | NiCl2 | Corynebacterium glutamicum | |
| Zn2+ | ZnSO4 | Corynebacterium glutamicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
sucrose | - |
Corynebacterium glutamicum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| arsenate | or phosphate is required | Corynebacterium glutamicum | |
| KCN | activates | Corynebacterium glutamicum | |
| NaN3 | activates | Corynebacterium glutamicum | |
| phosphate | or arsenate is required | Corynebacterium glutamicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 92000 | - |
gel filtration | Corynebacterium glutamicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Corynebacterium glutamicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 345 | - |
- |
Corynebacterium glutamicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| raffinose + H2O | 10% of the activity with sucrose | Corynebacterium glutamicum | fructose + melibiose | - |
? | |
| sucrose + H2O | - |
Corynebacterium glutamicum | D-fructose + D-glucose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Corynebacterium glutamicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Corynebacterium glutamicum |
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Release 2023.1 (January 2023)
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