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Literature summary for 3.2.1.26 extracted from

  • Williams, R.S.; Trumbly, R.J.; MacColl, R.; Trimble, R.B.; Maley, F.
    Comparative properties of amplified external and internal invertase from the yeast SUC2 gene (1985), J. Biol. Chem., 260, 13334-13341.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75760
-
pH 9.4, monomeric enzyme form, gel filtration Saccharomyces cerevisiae
112500
-
pH 8.3, dimeric enzyme form, gel filtration Saccharomyces cerevisiae
409000
-
pH 4.9, tetrameric enzyme form, gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein carbohydrate containing extracellular invertase and nonglycosylated intracellular form Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
extracellular and intracellular enzyme Saccharomyces cerevisiae

Renatured (Commentary)

Renatured (Comment) Organism
in the presence of carbohydrate considerably enhances the ability of guanidine HCl-denatured extracellular invertase to be renatured relative to intracellular invertase, after incubation with 2.5 M guanidine HCl for increasing periods of time. Less than 1 min is required for maximal renaturation for both extracellular and intracellular invertase Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + H2O
-
Saccharomyces cerevisiae D-fructose + D-glucose
-
?

Subunits

Subunits Comment Organism
More the intracellular enzyme exists as a monomer at pH 9.4, a dimer at pH 8.3, and an apparent octamer at pH 4.9 Saccharomyces cerevisiae