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Literature summary for 3.2.1.25 extracted from

  • Araujo, A.; Ward, O.
    Purification and some properties of the mannanases from Thielavia terrestris (1990), J. Ind. Microbiol., 6, 269-274 .
No PubMed abstract available
Search for more literature for 3.2.1.25

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Thermothielavioides terrestris
-
-

Organism

Organism UniProt Comment Textmining
Thermothielavioides terrestris
-
-
-
Thermothielavioides terrestris NRRL 8126
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein isoform M1, 18% carbohydrate content, isoform M2, 12% carbohydrate content, isoform M3, 6% carbohydrate content, isoform M4, 37% carbohydrate content Thermothielavioides terrestris

Purification (Commentary)

Purification (Comment) Organism
-
 Thermothielavioides terrestris

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
 Thermothielavioides terrestris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.17
-
 isoform M4, pH 5.6, 50°C Thermothielavioides terrestris
28.2
-
 isoform M1, pH 5.6, 50°C Thermothielavioides terrestris
38.7
-
 isoform M2, pH 5.6, 50°C Thermothielavioides terrestris
52.8
-
 isoform M3, pH 5.6, 50°C Thermothielavioides terrestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-mannopyranoside + H2O
-
 Thermothielavioides terrestris 4-nitrophenol + D-mannose
-
 ?
4-nitrophenyl beta-D-mannopyranoside + H2O
-
 Thermothielavioides terrestris NRRL 8126 4-nitrophenol + D-mannose
-
 ?
locust bean gum + H2O
-
 Thermothielavioides terrestris ?
-
 ?
locust bean gum + H2O
-
 Thermothielavioides terrestris NRRL 8126 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 52000, isoform M1, x * 30000, isoform M2, x * 55000, isoform M3, x * 89000, isoform M4, SDS-PAGE Thermothielavioides terrestris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 isoforms M1-M4, 3 h, no loss of activity Thermothielavioides terrestris
60
-
 isoform M4, 3 h, no loss of activity Thermothielavioides terrestris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5.5
-
 Thermothielavioides terrestris

Expression

Organism Comment Expression
Thermothielavioides terrestris expression upon culture on complex media containing locust bean gum up