Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Hordeum vulgare |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-nitrophenyl beta-D-thioglucoside | competitive, adopts 4NP-S-Glc 3S5 or 1S3 conformations upon binding according to STD NMR and trNOESY experiments. QM modeling and docking, based on GLIDE scores, predicts that 4-nitrophenyl beta-D-thioglucoside preferentially binds in 1S3 geometries | Hordeum vulgare | |
4-nitrophenyl beta-D-thiomannoside | competitive, adopts 4C1 geometry upon binding according to STD NMR and trNOESY experiments. QM modeling and docking, based on GLIDE scores, predicts that 4-nitrophenyl beta-D-thiomannoside preferentially binds in 1S3 geometries | Hordeum vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
4-nitrophenyl-beta-D-mannopyranose | pH 4.0, 30°C | Hordeum vulgare | |
0.5 | - |
4-nitrophenyl-beta-D-glucopyranose | pH 4.0, 30°C | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | B5A496 | var. distichum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranose + H2O | - |
Hordeum vulgare | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-mannopyranoside + H2O | - |
Hordeum vulgare | 4-nitrophenol + beta-D-mannose | - |
? | |
additional information | in glycoside recognition and substrate specificity of HvBII, a combination of the following determinants is likely to play key roles: the inherent conformational and spatial flexibilities of gluco- and manno-configured substrates in the enzymes' active sites, the subtle differences in the spatial disposition of active site residues and their capacities to form interactions with specific groups of substrates, and the small variations in the charge distributions and shapes of the catalytic sites | Hordeum vulgare | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HvBII | - |
Hordeum vulgare |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
4-nitrophenyl-beta-D-glucopyranose | pH 4.0, 30°C | Hordeum vulgare | |
3.1 | - |
4-nitrophenyl-beta-D-mannopyranose | pH 4.0, 30°C | Hordeum vulgare |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.095 | - |
4-nitrophenyl beta-D-thioglucoside | pH 4.0, 30°C | Hordeum vulgare | |
0.266 | - |
4-nitrophenyl beta-D-thiomannoside | pH 4.0, 30°C | Hordeum vulgare |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
4-nitrophenyl-beta-D-glucopyranose | pH 4.0, 30°C | Hordeum vulgare | |
12.7 | - |
4-nitrophenyl-beta-D-mannopyranose | pH 4.0, 30°C | Hordeum vulgare |