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Literature summary for 3.2.1.25 extracted from
- Purification and characterization of beta-mannosidases from white rot fungus Phlebia radiata (2007), Pol. J. Microbiol., 56, 139-147.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.29 | - |
p-nitrophenyl-beta-D-mannoside | isozyme GM-1 | Phlebia radiata |  |
| 0.34 | - |
p-nitrophenyl-beta-D-mannoside | isozyme OT-1 | Phlebia radiata | |
| 1.78 | - |
p-nitrophenyl-beta-D-mannoside | isozyme GM-2 | Phlebia radiata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 89500 | - |
isozyme GM-2, SDS-PAGE | Phlebia radiata |
| 100300 | - |
isozyme OT-1, SDS-PAGE | Phlebia radiata |
| 104600 | - |
isozyme GM-1, SDS-PAGE | Phlebia radiata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phlebia radiata | - |
- |
- |
| Phlebia radiata 79 / ATCC 64658 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose column chromatography, SP-Sepharose column chromatography, and chromatofocusing on Polybuffer Exchanger PBE-94 | Phlebia radiata |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30.7 | - |
supernatant | Phlebia radiata |
| 3732 | - |
after 121.55fold purification | Phlebia radiata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl-beta-D-mannoside + H2O | - |
Phlebia radiata | p-nitrophenol + alpha-D-mannose | - |
? | |
| p-nitrophenyl-beta-D-mannoside + H2O | - |
Phlebia radiata 79 / ATCC 64658 | p-nitrophenol + alpha-D-mannose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GM-1 | isozyme | Phlebia radiata |
| GM-2 | isozyme | Phlebia radiata |
| OT-1 | isozyme | Phlebia radiata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Phlebia radiata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Phlebia radiata |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Phlebia radiata | isozyme GM-2, isoelectric focusing | - |
3.8 |
| Phlebia radiata | isozyme OT-1, isoelectric focusing | - |
4.65 |
| Phlebia radiata | isozyme GM-1, isoelectric focusing | - |
4.8 |
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