Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Cellvibrio mixtus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method | Cellvibrio mixtus |
Protein Variants | Comment | Organism |
---|---|---|
E330A | mutant enzyme displays no activity against mannan or 4-nitrophenyl-beta-mannopyranoside | Cellvibrio mixtus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000083 | - |
4-nitrophenyl-beta-mannopyranose | pH 7.0, 37°C, mutant enzyme E215A | Cellvibrio mixtus | |
0.1 | - |
Mannotriose | pH 7.0, 37°C, wild-type enzyme | Cellvibrio mixtus | |
0.9 | - |
mannobiose | pH 7.0, 37°C, wild-type enzyme | Cellvibrio mixtus | |
1.6 | - |
4-nitrophenyl-beta-D-mannopyranose | pH 7.0, 37°C, wild-type enzyme | Cellvibrio mixtus | |
6.1 | - |
beta-D-mannosyl-1,4-beta-D-glucoside | pH 7.0, 37°C, wild-type enzyme | Cellvibrio mixtus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellvibrio mixtus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Cellvibrio mixtus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-galactosyl-mannotriose + H2O | - |
Cellvibrio mixtus | ? | - |
? | |
4-nitrophenyl beta-D-mannopyranoside + H2O | - |
Cellvibrio mixtus | 4-nitrophenol + alpha-D-mannose | - |
? | |
4-O-beta-D-mannopyranosyl-D-mannopyranose + H2O | - |
Cellvibrio mixtus | D-mannopyranose | - |
? | |
beta-D-mannosyl-1,4-beta-D-glucoside + H2O | - |
Cellvibrio mixtus | D-mannose + beta-D-glucose | - |
? | |
mannobiose + H2O | - |
Cellvibrio mixtus | 2 D-mannopyranose | - |
? | |
mannotetraose + H2O | - |
Cellvibrio mixtus | ? | - |
? | |
mannotriose + H2O | - |
Cellvibrio mixtus | ? | - |
? | |
additional information | CmMan5A releases beta-1,4-linked mannose substrates exclusively from the nonreducing end | Cellvibrio mixtus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CmMan5a | - |
Cellvibrio mixtus |
mannosidase 5A | - |
Cellvibrio mixtus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
4-nitrophenyl-beta-D-mannopyranose | pH 7.0, 37°C, wild-type enzyme | Cellvibrio mixtus | |
0.053 | - |
beta-D-mannosyl-1,4-beta-D-glucoside | pH 7.0, 37°C, wild-type enzyme | Cellvibrio mixtus | |
0.1 | - |
4-nitrophenyl-beta-mannopyranose | pH 7.0, 37°C, mutant enzyme E215A | Cellvibrio mixtus | |
2.17 | - |
4-O-beta-D-mannopyranosyl-D-mannopyranose | pH 7.0, 37°C, wild-type enzyme | Cellvibrio mixtus | |
166.7 | - |
Mannotriose | pH 7.0, 37°C, wild-type enzyme | Cellvibrio mixtus |