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Literature summary for 3.2.1.25 extracted from

  • Mo, B.; Bewley, J.D.
    beta-Mannosidase (EC 3.2.1.25) activity during and following germination of tomato (Lycopersicon esculentum Mill.) seeds. Purification, cloning and characterization (2002), Planta, 215, 141-152.
    View publication on PubMed
Search for more literature for 3.2.1.25

Cloned(Commentary)

Cloned (Comment) Organism
-
 Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.55
-
 p-nitrophenyl beta-D-mannopyranoside
-
 Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall ionically associated with a matrix fraction, most likely the cell wall Solanum lycopersicum 5618
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 x * 56000, SDS-PAGE Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Solanum lycopersicum coincidental and substantial increase in activity of both beta-mannosidase and beta-mannanase after 48 h, following germination, suggests the cooperation of both enzymes in the degradation of endosperm cell wall galactomannan ?
-
 ?
additional information Solanum lycopersicum Mill. coincidental and substantial increase in activity of both beta-mannosidase and beta-mannanase after 48 h, following germination, suggests the cooperation of both enzymes in the degradation of endosperm cell wall galactomannan ?
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
 Mill.
-
Solanum lycopersicum Mill.
-
 Mill.
-

Purification (Commentary)

Purification (Comment) Organism
-
 Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
seed increase in activity following germination Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.307
-
-
 Solanum lycopersicum

Storage Stability

Storage Stability Organism
-80°C, pH 5.0, chromatofocusing elution buffer, loss of activity within 1 month Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information coincidental and substantial increase in activity of both beta-mannosidase and beta-mannanase after 48 h, following germination, suggests the cooperation of both enzymes in the degradation of endosperm cell wall galactomannan Solanum lycopersicum ?
-
 ?
additional information coincidental and substantial increase in activity of both beta-mannosidase and beta-mannanase after 48 h, following germination, suggests the cooperation of both enzymes in the degradation of endosperm cell wall galactomannan Solanum lycopersicum Mill. ?
-
 ?
p-nitrophenyl beta-D-mannopyranoside + H2O
-
 Solanum lycopersicum p-nitrophenol + beta-D-mannopyranose
-
 ?
p-nitrophenyl beta-D-mannopyranoside + H2O
-
 Solanum lycopersicum Mill. p-nitrophenol + beta-D-mannopyranose
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Solanum lycopersicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Solanum lycopersicum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 55 40°C: optimum, at optimal pH active up to 55°C Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Solanum lycopersicum