Literature summary for 3.2.1.25 extracted from

Parker, K.N.; Chhabra, S.; Lam, D.; Snead, M.A.; Mathur, E.J.; Kelly, R.M.
beta-Mannosidase from Thermotoga species (2001), Methods Enzymol., 330, 238-246.

Search for more literature for 3.2.1.25

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.1	-	p-nitrophenyl beta-D-mannopyranoside	-	Thermotoga neapolitana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
intracellular	-	Thermotoga neapolitana	5622	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
95000	-	2 * 95000, SDS-PAGE	Thermotoga neapolitana
200000	-	-	Thermotoga neapolitana

Organism

Organism	UniProt	Comment	Textmining
Thermotoga neapolitana	-	-	-
Thermotoga neapolitana 5068	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermotoga neapolitana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
34	-	-	Thermotoga neapolitana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
p-nitrophenyl beta-D-mannopyranoside + H2O	-	Thermotoga neapolitana	p-nitrophenol + beta-D-mannose	-	?
p-nitrophenyl beta-D-mannopyranoside + H2O	-	Thermotoga neapolitana 5068	p-nitrophenol + beta-D-mannose	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 95000, SDS-PAGE	Thermotoga neapolitana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
87	-	-	Thermotoga neapolitana

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
85	-	half-life: 18 h	Thermotoga neapolitana
90	-	half-life: 42 min	Thermotoga neapolitana
98	-	half-life: 2 min	Thermotoga neapolitana

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
67.8	-	p-nitrophenyl beta-D-mannopyranoside	-	Thermotoga neapolitana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.7	-	-	Thermotoga neapolitana

pI Value

Organism	Comment	pI Value Maximum	pI Value
Thermotoga neapolitana	-	-	6

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Release 2023.1 (January 2023)