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Literature summary for 3.2.1.25 extracted from
- Galactomannan structure and beta-mannanase and beta-mannosidase activity in germinating legume seeds (1975), Phytochemistry, 14, 1187-1194.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | - |
Medicago sativa |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.29 | - |
p-nitrophenyl-beta-D-mannoside | enzyme from endosperm | Ceratonia siliqua | |
| 0.29 | - |
p-nitrophenyl-beta-D-mannoside | enzyme from cotyledons | Medicago sativa | |
| 0.29 | - |
p-nitrophenyl-beta-D-mannoside | enzyme from cotyledons | Ceratonia siliqua | |
| 0.83 | - |
p-nitrophenyl-beta-D-mannoside | enzyme from endosperm | Medicago sativa | |
| 1.2 | - |
mannotriitol | enzyme from endosperm | Medicago sativa | |
| 10 | - |
mannotriitol | enzyme from cotyledons | Medicago sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
enzyme from cotyledons, gel filtration | Medicago sativa |
| 54000 | - |
enzyme from cotyledons, gel filtration | Ceratonia siliqua |
| 100000 | - |
enzyme from endosperm, gel filtration | Medicago sativa |
| 100000 | - |
enzyme from endosperm, gel filtration | Ceratonia siliqua |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ceratonia siliqua | - |
- |
- |
| Medicago sativa | - |
subsp. sativa | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | enzyme form A | Medicago sativa | - |
| cotyledon | enzyme form A | Ceratonia siliqua | - |
| endosperm | enzyme form B | Medicago sativa | - |
| endosperm | enzyme form B | Ceratonia siliqua | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-mannotriitol + H2O | - |
Medicago sativa | ? | - |
? | |
| mannotriitol + H2O | - |
Medicago sativa | ? | - |
? | |
| p-nitrophenyl-beta-D-mannoside + H2O | - |
Medicago sativa | p-nitrophenol + alpha-D-mannose | - |
? | |
| p-nitrophenyl-beta-D-mannoside + H2O | - |
Ceratonia siliqua | p-nitrophenol + alpha-D-mannose | - |
? | |
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