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Literature summary for 3.2.1.25 extracted from

  • Leipprandt, J.R.; Kraemer, S.A.; Haithcock, B.E.; Chen, H.; Dyme, J.L.; Cavanagh, K.T.; Fridericic, K.H.; Jones, M.Z.
    Caprine beta-mannosidase: sequencing and characterization of the cDNA and identification of the molecular defect of caprine beta-mannosidosis (1996), Genomics, 37, 51-56.
    View publication on PubMed
Search for more literature for 3.2.1.25

Protein Variants

Protein Variants Comment Organism
additional information mutation associated with caprine beta-mannosidosis: single-base deletion at position 1398 of the coding sequence results in a shift in the reading frame, yielding a deduced peptide of 481 amino acids Capra hircus

General Stability

General Stability Organism
Stable to freeze-drying Trifolium repens
stable to freezing, -20°C, and thawing Trifolium repens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 beta-mannanase II, disc gel electrophoresis Trifolium repens
43000
-
 beta-mannanase I, disc gel electrophoresis Trifolium repens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Capra hircus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Capra hircus Q95327
-
-
Trifolium repens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
beta-mannanase I and II Trifolium repens

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Capra hircus
-
seed germinated Trifolium repens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
32.86
-
 beta-mannanase II Trifolium repens
45.61
-
 beta-mannanase I Trifolium repens

Storage Stability

Storage Stability Organism
-20°C, freeze-dried enzyme, stable Trifolium repens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information
-
 Capra hircus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 38000, beta-mannanase II, SDS-PAGE Trifolium repens
monomer 1 * 43000, beta-mannanase I, SDS-PAGE Trifolium repens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 60 min, beta-mannanase I and II, stable Trifolium repens
60
-
 complete inactivation of beta-mannanase I after 60 min, complete inactivation of beta-mannanase II after 40 min Trifolium repens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1 5.6
-
 Trifolium repens