Literature summary for 3.2.1.24 extracted from

Retamal, C.A.; Dias, A.J.; Brasil, F.C.; Lanzana, F.R.; Lopez, M.L.
Alpha-mannosidase activity in stallion epididymal fluid and spermatozoa (2012), Theriogenology, 78, 252-262.

Search for more literature for 3.2.1.24

Inhibitors

Inhibitors	Comment	Organism	Structure
swainsonine	swainsonine treatment completely inhibits the acid enzyme form activity below pH 5.0	Equus caballus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.95	-	4-methylumbelliferyl alpha-D-mannopyranoside	at pH 5.0 and 37°C	Equus caballus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Equus caballus	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	the neutral enzyme form is activated by Co2+	Equus caballus
additional information	the acid enzyme form is not activated by Co2+	Equus caballus
Zn2+	the acid enzyme form is stabilized or stimulated by Zn2+	Equus caballus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
154000	-	gel filtration	Equus caballus

Organism

Organism	UniProt	Comment	Textmining
Equus caballus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
epididymal fluid	the activity of acid alpha-mannosidase I is highest in the epididymal fluid	Equus caballus	-
spermatozoon	the activity of neutral alpha-mannosidase II is highest in mature spermatozoa	Equus caballus	-

Storage Stability

Storage Stability	Organism
-20°C, 7, 10 and 30 days, 20%, 30% and 40% loss of activity, respectively	Equus caballus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl alpha-D-mannopyranoside + H2O	-	Equus caballus	4-methylumbelliferone + alpha-D-mannopyranose	-	?

Synonyms

Synonyms	Comment	Organism
alpha-1,4 mannosidase	-	Equus caballus
alpha-D-mannosidase	-	Equus caballus
alpha-mannosidase I	acid enzyme form	Equus caballus
alpha-mannosidase II	neutral enzyme form	Equus caballus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	80% of initial activity remains after 40-min incubation time at 60°C. After 60 min at this temperature, activity is lost	Equus caballus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.5	4	isoform alpha-mannosidase I	Equus caballus

General Information

General Information	Comment	Organism
physiological function	the acid enzyme form alpha-mannosidase I plays a role in plasma membrane remodeling associated with sperm maturation. The neutral enzyme form alpha-mannosidase II acts as a receptor in the recognition and binding of the complementary carbohydrate moieties present on the zona pellucida	Equus caballus

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Release 2023.1 (January 2023)