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Literature summary for 3.2.1.23 extracted from

  • Nakkharat, P.; Haltrich, D.
    Lactose hydrolysis and formation of galactooligosaccharides by a novel immobilized beta-galactosidase from the thermophilic fungus Talaromyces thermophilus (2006), Appl. Biochem. Biotechnol., 129-132, 215-225 .
    View publication on PubMed
Search for more literature for 3.2.1.23

Application

Application Comment Organism
synthesis immobilization by covalent attachment onto Eupergit C with a binding efficiency of 95%. Immobilization increases both activity and stability at higher pH values and temperature but does not significantly change kinetic parameters for the substrate lactose. The immobilized enzyme shows a strong transgalactosylation reaction, resulting in the formation of galactooligosaccharides. The maximum yield of 34% galactooligosaccharides is obtained when the degree of lactose conversion is roughly 80% Thermomyces dupontii

Inhibitors

Inhibitors Comment Organism Structure
D-galactose
-
 Thermomyces dupontii
D-glucose
-
 Thermomyces dupontii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11
-
 2-nitrophenyl beta-D-galactoside immobilized enzyme, pH 6.5, 40°C Thermomyces dupontii
19
-
 lactose native enzyme, pH 6.5, 40°C Thermomyces dupontii
21
-
 lactose immobilized enzyme, pH 6.5, 40°C Thermomyces dupontii
35
-
 2-nitrophenyl beta-D-galactoside native enzyme, pH 6.5, 40°C Thermomyces dupontii

Organism

Organism UniProt Comment Textmining
Thermomyces dupontii
-
-
-
Thermomyces dupontii CBS 236.58
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactoside + H2O
-
 Thermomyces dupontii 2-nitrophenol + beta-D-galactose
-
 ?
2-nitrophenyl beta-D-galactoside + H2O
-
 Thermomyces dupontii CBS 236.58 2-nitrophenol + beta-D-galactose
-
 ?
lactose + H2O
-
 Thermomyces dupontii D-galactose + D-glucose
-
 ?
lactose + H2O
-
 Thermomyces dupontii CBS 236.58 D-galactose + D-glucose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 native enzyme, substrate 2-nitrophenyl beta-D-galactoside Thermomyces dupontii
50
-
 immobilized enzyme, substrate 2-nitrophenyl beta-D-galactoside Thermomyces dupontii
50
-
 native enzyme, substrate lactose Thermomyces dupontii
60
-
 immobilized enzyme, substrate lactose Thermomyces dupontii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-life 8 h for native enzyme, 27 h for immobilized enzyme, respectively Thermomyces dupontii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 native enzyme, substrate lactose Thermomyces dupontii
6
-
 immobilized enzyme, and native enzyme with substrate 2-nitrophenyl beta-D-galactoside Thermomyces dupontii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
58
-
 D-glucose immobilized enzyme, pH 6.5, 40°C Thermomyces dupontii
66
-
 D-glucose native enzyme, pH 6.5, 40°C Thermomyces dupontii
251
-
 D-galactose immobilized enzyme, 2-nitrophenyl beta-D-galactoside, pH 6.5, 40°C Thermomyces dupontii
355
-
 D-galactose immobilized enzyme, substrate lactose, pH 6.5, 40°C Thermomyces dupontii
370
-
 D-galactose native enzyme, substrate 2-nitrophenyl beta-D-galactoside, pH 6.5, 40°C Thermomyces dupontii
420
-
 D-galactose native enzyme, substrate lactose, pH 6.5, 40°C Thermomyces dupontii