Cloned (Comment) | Organism |
---|---|
expressed in the genetically tractable haloarchaeon Halobacterium sp. NRC-1 under the control of a cold shock protein (cspD2) gene promoter | Halorubrum lacusprofundi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ethanol | 10% v/v, about 40% loss of activity | Halorubrum lacusprofundi | |
isoamyl alcohol | 10% v/v, about 35% loss of activity | Halorubrum lacusprofundi | |
methanol | 10% v/v, about 5% loss of activity | Halorubrum lacusprofundi | |
n-butanol | 10% v/v, about 35% loss of activity | Halorubrum lacusprofundi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | maximal activity in either 4 M NaCl or KCl | Halorubrum lacusprofundi | |
NaCl | maximal activity in either 4 M NaCl or KCl | Halorubrum lacusprofundi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78060 | - |
x * 78060, calculated from sequence | Halorubrum lacusprofundi |
100000 | - |
x * 100000, SDS-PAGE | Halorubrum lacusprofundi |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | 10-20% v/v, 34% loss of activity after the first h and 25-30% after 3 hours | Halorubrum lacusprofundi |
isoamyl alcohol | 10-20% v/v, 34% loss of activity after the first h and 25-30% after 3 hours | Halorubrum lacusprofundi |
Methanol | 10-20% v/v, 34% loss of activity after the first h and 25-30% after 3 hours | Halorubrum lacusprofundi |
n-Butanol | 10-20% v/v, 34% loss of activity after the first h and 25-30% after 3 hours | Halorubrum lacusprofundi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halorubrum lacusprofundi | B9LW38 | - |
- |
Halorubrum lacusprofundi DSM 5036 | B9LW38 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Halorubrum lacusprofundi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
110.8 | - |
pH 6.0, 25°C | Halorubrum lacusprofundi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Halorubrum lacusprofundi | 2-nitrophenol + beta-D-galactose | - |
? | |
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Halorubrum lacusprofundi DSM 5036 | 2-nitrophenol + beta-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 100000, SDS-PAGE | Halorubrum lacusprofundi |
? | x * 78060, calculated from sequence | Halorubrum lacusprofundi |
Synonyms | Comment | Organism |
---|---|---|
Hlac_2868 | locus name | Halorubrum lacusprofundi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Halorubrum lacusprofundi |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 60 | 35°C: about 55% of maximal activity, 60°C: about 80% of maximal activity | Halorubrum lacusprofundi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Halorubrum lacusprofundi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | pH 6.0: about 60% of maximal activity, pH 7.5: about 40% of maximal activity | Halorubrum lacusprofundi |