Application | Comment | Organism |
---|---|---|
food industry | the recombinant thermostable beta-galactosidase may be suitable for the hydrolysis of lactose in milk processing | Pyrococcus furiosus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21. The active inclusion bodies of beta-galactosidase are easily isolated by nonionic detergent treatment and directly used for lactose conversion in a repetitive batch mode | Pyrococcus furiosus |
General Stability | Organism |
---|---|
when the recombinant enzyme from active inclusion bodies is utilized for lactose conversion in a repetitive batch mode, more than 54% (90°C) or 88% (10°C) of the original enzyme activity is retained after 10 conversion cycles under optimum conditions | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, inhibits the enzyme activity by 22.7%, 80°C, pH 7.0 | Pyrococcus furiosus | |
Cu2+ | 5 mM, inhibits the enzyme activity by 99.71%, 80°C, pH 7.0 | Pyrococcus furiosus | |
Fe3+ | 5 mM, inhibits the enzyme activity by 95.2%, 80°C, pH 7.0 | Pyrococcus furiosus | |
Mn2+ | 5 mM, inhibits the enzyme activity by 24.8%, 80°C, pH 7.0 | Pyrococcus furiosus | |
Ni2+ | 5 mM, inhibits the enzyme activity by 92.9%, 80°C, pH 7.0 | Pyrococcus furiosus | |
Zn2+ | 5 mM, inhibits the enzyme activity by 97.3%, 80°C, pH 7.0 | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5 mM, 1.1fold stimulation of activity, 80°C, pH 7.0 | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59450 | - |
x * 59450, SDS-PAGE | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | E7FHC0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.2 | - |
pH 7, 0°C | Pyrococcus furiosus |
130 | - |
pH 7, 90°C | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Pyrococcus furiosus | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | 94% of the activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pyrococcus furiosus | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
lactose + H2O | - |
Pyrococcus furiosus | D-glucose + D-galactose | - |
? | |
additional information | activity with 4-nitrophenyl alpha-D-glucopyranoside is 0.26% of the activity compared to 2-nitrophenyl beta-D-galactopyranoside, activity with 4-nitrophenyl alpha-D-glucopyranoside is 10.91% of the activity compared to 2-nitrophenyl beta-D-galactopyranoside | Pyrococcus furiosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 59450, SDS-PAGE | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
lactase | - |
Pyrococcus furiosus |
PF1208 | locus name | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 100 | 60°C: about 50% of maximal activity, 100°C: about 95% of maximal activity | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
pH 7.0, half-life: 31423.4 min | Pyrococcus furiosus |
80 | - |
pH 7.0, half-life: 8168.3 min | Pyrococcus furiosus |
85 | - |
pH 7.0, half-life: 4017.7 min | Pyrococcus furiosus |
90 | - |
pH 7.0, half-life: 547.4 min | Pyrococcus furiosus |
95 | - |
pH 7.0, half-life: 309.6 min | Pyrococcus furiosus |
100 | - |
pH 7.0, half-life: 203.5 min | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
in phosphate buffer | Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | pH 6.5: about 60% of maximal activity, pH 7.5: about 50% of maximal activity | Pyrococcus furiosus |