Application | Comment | Organism |
---|---|---|
synthesis | the enzyme catalyzes the production of the synthetic disaccharide lactulose (4-O-beta-D-galactopyranosyl-D-fructose) via a transgalactosylation using lactose as a galactose donor and fructose as an acceptor. Lactulose is used in treatment of hyperammonemia and as a gentle laxative. It is also applied to commercial infant formulas and various milk products because it specifically promotes the intestinal proliferation of Bifidobacterium, which creates an acid medium that inhibits the growth of undesirable bacteria | Saccharolobus solfataricus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a soluble protein that contained a hexa-histidine tag at the carboxyl terminus | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | increases activity | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56561 | - |
x * 56561, calculated from sequence | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | Saccharolobus solfataricus | - |
D-glucose + D-galactose | - |
? | |
lactose + H2O | Saccharolobus solfataricus DSM 1617 | - |
D-glucose + D-galactose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | P22498 | - |
- |
Saccharolobus solfataricus DSM 1617 | P22498 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | - |
Saccharolobus solfataricus | D-glucose + D-galactose | - |
? | |
lactose + H2O | - |
Saccharolobus solfataricus DSM 1617 | D-glucose + D-galactose | - |
? | |
additional information | the enzyme catalyzes the production of the synthetic disaccharide lactulose (4-O-beta-D-galactopyranosyl-D-fructose) via a transgalactosylation using lactose as a galactose donor and fructose as an acceptor | Saccharolobus solfataricus | ? | - |
? | |
additional information | the enzyme catalyzes the production of the synthetic disaccharide lactulose (4-O-beta-D-galactopyranosyl-D-fructose) via a transgalactosylation using lactose as a galactose donor and fructose as an acceptor | Saccharolobus solfataricus DSM 1617 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56561, calculated from sequence | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
half-life: 91 h | Saccharolobus solfataricus |
80 | - |
half-life: 48 h | Saccharolobus solfataricus |
85 | - |
half-life: 35 h | Saccharolobus solfataricus |
90 | - |
half-life: 2.6 h | Saccharolobus solfataricus |
95 | - |
half-life: 0.72 h | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | pH 4.0: about 45% of maximal activity, pH 8.0: about 80% of maximal activity | Saccharolobus solfataricus |