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Literature summary for 3.2.1.23 extracted from

  • Xu, K.; Tang, X.; Gai, Y.; Mehmood, M.; Xiao, X.; Wang, F.
    Molecular characterization of cold-inducible beta-galactosidase from Arthrobacter sp. ON14 isolated from Antarctica (2011), J. Microbiol. Biotechnol., 21, 236-242.
    View publication on PubMed
Search for more literature for 3.2.1.23

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ inactivates enzymatic activity Arthrobacter sp.
Zn2+ reduces enzymatic activity Arthrobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates enzymatic activity Arthrobacter sp.
Mn2+ activates enzymatic activity Arthrobacter sp.
Na+ activates enzymatic activity Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
116000
-
 SDS-PAGE Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp. D9IU57 ON14
-
Arthrobacter sp. D9IU58 ON14
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Arthrobacter sp. 2-nitrophenol + beta-D-galactose
-
 ?
5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside + H2O
-
 Arthrobacter sp. 5-bromo-4-chloro-indol + beta-D-galactose
-
 ?

Synonyms

Synonyms Comment Organism
beta-galactosidase
-
 Arthrobacter sp.
GALA
-
 Arthrobacter sp.
GALB
-
 Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15
-
-
 Arthrobacter sp.
37
-
-
 Arthrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 50 an enzyme stability assay reveals that the activity of GalA is significantly decreased when it is incubated at 45°C for 2 h, and all its activity is lost when it is incubated at 50°C Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 9 75% activity Arthrobacter sp.

Expression

Organism Comment Expression
Arthrobacter sp. expression is inhibited at low temperature down
Arthrobacter sp. expression is induced at high temperature up
Arthrobacter sp. expression of galA is highly induced at a low temperature and repressed at a high temperature when lactose is used as the sole carbon source up