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Literature summary for 3.2.1.23 extracted from

  • Schmidt, M.; Stougaard, P.
    Identification, cloning and expression of a cold-active beta-galactosidase from a novel Arctic bacterium, Alkalilactibacillus ikkense (2010), Environ. Technol., 31, 1107-1114.
    View publication on PubMed
Search for more literature for 3.2.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Alkalilactibacillus ikkensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
 denaturating gel-electrophoresis of both recombinant and native protein show a molecular weight of 115-120 kDa Alkalilactibacillus ikkensis

Organism

Organism UniProt Comment Textmining
Alkalilactibacillus ikkensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Alkalilactibacillus ikkensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information when lactose is present in substantial amounts, the enzyme displays transgalactosylation activity Alkalilactibacillus ikkensis ?
-
 ?

Synonyms

Synonyms Comment Organism
beta-galactosidase
-
 Alkalilactibacillus ikkensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 enzyme highly active at low temperatures: 60% at 0°C Alkalilactibacillus ikkensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 30 optimal activity Alkalilactibacillus ikkensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 40 stable for 100 hours at and below 10°C. At temperatures at and above 40°C enzyme is inactivated within 10 min Alkalilactibacillus ikkensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Alkalilactibacillus ikkensis