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Literature summary for 3.2.1.23 extracted from

  • Stano, J.; Siekel, P.; Neubert, K.; Micieta, K.
    Study of gherkin lactase in cell culture and in seedlings (2011), Acta Histochem., 113, 631-635.
    View publication on PubMed
Search for more literature for 3.2.1.23

Inhibitors

Inhibitors Comment Organism Structure
D-galactose 2% residual activity at 50 mM and 86% residual activity at 0.1 mM (purified lactase) Cucumis sativus
glucose 82% residual activity at 100 mM and 95% residual activity at 20 mM (purified lactase) Cucumis sativus
lactose 92% residual activity at 50 mM and 97% residual activity at 20 mM (purified lactase) Cucumis sativus
melibiose 44% residual activity at 100 mM and 86% residual activity at 0.1 mM (purified lactase); 44% residual activity at 100 mM and 86% residual activity at 10 mM (purified lactase) Cucumis sativus
additional information not inhibited by fructose, maltose and saccharose Cucumis sativus
raffinose 53% residual activity at 200 mM and 95% residual activity at 20 mM (purified lactase) Cucumis sativus
stachyose 90% residual activity at 100 mM and 79% residual activity at 10 mM (purified lactase) Cucumis sativus

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Cucumis sativus
-
 cultivar Palava
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Sephadex G-100 gel filtration Cucumis sativus

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Cucumis sativus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.025
-
 crude homogenate, at pH 4.8, 60°C Cucumis sativus
0.27
-
 after 11.1fold purification, at pH 4.8, 60°C Cucumis sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl beta-D-galactopyranoside + H2O
-
 Cucumis sativus 1-naphthol + beta-D-galactopyranose
-
 ?
2-nitrophenyl beta-D-galactopyranoside + H2O The enzyme hydrolysis of 2-nitrophenyl beta-D-galactopyranoside is linear within 3.5 h reaching 60-65% of substrate conversion, than practically stops Cucumis sativus 2-nitrophenol + beta-D-galactopyranose
-
 ?
lactose + H2O
-
 Cucumis sativus D-galactose + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
gherkin lactase
-
 Cucumis sativus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
56
-
 in permeabilized cell suspension Cucumis sativus
60
-
 in immobilized cell suspension Cucumis sativus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
-
 Cucumis sativus