Literature summary for 3.2.1.23 extracted from

Fujimoto, A.; Hirano, A.; Shiraki, K.
Ternary system of solution additives with arginine and salt for refolding of beta-galactosidase (2010), Protein J., 29, 161-166.

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Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
lactose + H2O	Escherichia coli	-	D-galactose + D-glucose	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Renatured (Commentary)

Renatured (Comment)	Organism
refolding buffer (pH 8.0) contains 10 mM MgCl2, 0.01% Tween 20, 2.5 mM dithiothreitol, and 100 mM Tris-HCl. In a binary system using only L-Arg-HCl the refolding yield of beta-gal increases with increasing concentration up to 0.2 M, in a ternary system using both 0.2 M L-Arg-HCl and another salt, the refolding yield increases up to 1.5fold higher than that in the binary system	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O	-	Escherichia coli	2-nitrophenol + beta-D-galactose	-	?
lactose + H2O	-	Escherichia coli	D-galactose + D-glucose	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 000, SDS-PAGE	Escherichia coli

Synonyms

Synonyms	Comment	Organism
beta-gal	-	Escherichia coli

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Release 2023.1 (January 2023)