Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris GS115 cells | Bispora sp. |
General Stability | Organism |
---|---|
the enzyme is strongly resistant to pepsin and trypsin digestion, after incubation in SGF at 37°C for 60 min recombinant BgalA retains almost all of its activity at the whole stomach stage with or without the presence of pepsin | Bispora sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 41.5% residual activity at 10 mM | Bispora sp. | |
EDTA | 86.2% residual activity at 10 mM | Bispora sp. | |
Mn2+ | 90.1% residual activity at 5 mM | Bispora sp. | |
SDS | 7.2% residual activity at 5 mM | Bispora sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
lactose | in glycine-HCl buffer (pH 1.5) | Bispora sp. | |
5.22 | - |
2-nitrophenyl-beta-D-galactopyranoside | in glycine-HCl buffer (pH 1.5) | Bispora sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 108.7% relative activity at 10 mM | Bispora sp. | |
Co2+ | 109.1% relative activity at 10 mM | Bispora sp. | |
Cr3+ | 115.0% relative activity at 2 mM | Bispora sp. | |
Cu2+ | 110.1% relative activity at 10 mM | Bispora sp. | |
Fe3+ | 104.4.4% relative activity at 10 mM | Bispora sp. | |
Hg2+ | 110.4% relative activity at 2 mM | Bispora sp. | |
K+ | 107.7% relative activity at 10 mM | Bispora sp. | |
Li+ | 110.1% relative activity at 10 mM | Bispora sp. | |
additional information | not stimulated by Mg2+ and Mn2+ | Bispora sp. | |
Na+ | 105.3% relative activity at 2 mM | Bispora sp. | |
Ni2+ | 103.4% relative activity at 2 mM | Bispora sp. | |
Pb2+ | 105.6% relative activity at 2 mM | Bispora sp. | |
Zn2+ | 105.2% relative activity at 10 mM | Bispora sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
calculated from amino acid sequence | Bispora sp. |
130000 | - |
gel filtration | Bispora sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | Bispora sp. | under simulated gastric conditions, BgalA has greater stability (about 100%) and hydrolysis ratio (more than 80%) toward milk lactose than the commercially available beta-galactosidase from Aspergillus oryzae (ATCC 20423) | D-galactose + D-glucose + oligosaccharide | the main products of lactose hydrolysis by recombinant BgalA are 19.5% D-galactose, 72.5% D-glucose, and 8.0% oligosaccharide | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bispora sp. | C7SLY9 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Bispora sp. |
Purification (Comment) | Organism |
---|---|
Q sepharose column chromatography | Bispora sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Bispora sp. | 2-nitrophenol + beta-D-galactose | - |
? | |
lactose + H2O | under simulated gastric conditions, BgalA has greater stability (about 100%) and hydrolysis ratio (more than 80%) toward milk lactose than the commercially available beta-galactosidase from Aspergillus oryzae (ATCC 20423) | Bispora sp. | D-galactose + D-glucose + oligosaccharide | the main products of lactose hydrolysis by recombinant BgalA are 19.5% D-galactose, 72.5% D-glucose, and 8.0% oligosaccharide | ? |
Synonyms | Comment | Organism |
---|---|---|
BgalA | - |
Bispora sp. |
lactase | - |
Bispora sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bispora sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 80 | the enzyme retains more than 40% of maximum activity between 37 and 80°C | Bispora sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | no activity is lost after incubation at 60°C for 60 min and more than 60% of the initial activity is retained after incubation at 70°C for 60 min, most of the enzymatic activity is lost within 2 min at 80°C | Bispora sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
1.5 | - |
- |
Bispora sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
0.5 | 8 | purified recombinant BgalA has measurable enzymatic activity over a pH range of 0.5 through 8.0 (measured at 37°C) | Bispora sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
0.5 | 12 | the enzyme retains more than 50% of its initial activity after incubation at pH 0.5 through 12.0 for 60 min | Bispora sp. |