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Literature summary for 3.2.1.23 extracted from

  • Gantulga, D.; Turan, Y.; Bevan, D.R.; Esen, A.
    The Arabidopsis At1g45130 and At3g52840 genes encode beta-galactosidases with activity toward cell wall polysaccharides (2008), Phytochemistry, 69, 1661-1670.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall associated with the cell wall Arabidopsis thaliana 5618
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
x * 79000, calculated, x * 75000, SDS-PAGE Arabidopsis thaliana
75000
-
x * 81000, calculated, x * 75000, SDS-PAGE Arabidopsis thaliana
79000
-
x * 79000, calculated, x * 75000, SDS-PAGE Arabidopsis thaliana
81000
-
x * 81000, calculated, x * 75000, SDS-PAGE Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9LFA6
-
-
Arabidopsis thaliana Q9MAJ7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
additional information low expression in root and silique Arabidopsis thaliana
-
rosette leaf
-
Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
Arabidopsis thaliana 2-nitrophenol + D-galactose 61% of the activity with 4-nitrophenyl-beta-D-galactopyranoside ?
2-nitrophenyl beta-D-galactopyranoside + H2O
-
Arabidopsis thaliana 2-nitrophenol + D-galactose 72% of the activity with 4-nitrophenyl-beta-D-galactopyranoside ?
4-methylumbelliferyl-beta-D-galactoside + H2O
-
Arabidopsis thaliana 4-methylumbelliferone + beta-D-galactose 18% of the activity with 4-nitrophenyl-beta-D-galactopyranoside ?
4-methylumbelliferyl-beta-D-galactoside + H2O
-
Arabidopsis thaliana 4-methylumbelliferone + beta-D-galactose 20% of the activity with 4-nitrophenyl-beta-D-galactopyranoside ?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
Arabidopsis thaliana 4-nitrophenol + D-galactose
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
-
Arabidopsis thaliana 5-bromo-4-chloroindol + D-galactose 43% of the activity with 4-nitrophenyl-beta-D-galactopyranoside ?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
-
Arabidopsis thaliana 5-bromo-4-chloroindol + D-galactose 56% of the activity with 4-nitrophenyl-beta-D-galactopyranoside ?
6-bromo-2-naphthyl-beta-galactoside + H2O
-
Arabidopsis thaliana 6-bromo-2-naphthol + D-galactose 22% of the activity with 4-nitrophenyl-beta-D-galactopyranoside ?
6-bromo-2-naphthyl-beta-galactoside + H2O
-
Arabidopsis thaliana 6-bromo-2-naphthol + D-galactose 30% of the activity with 4-nitrophenyl-beta-D-galactopyranoside ?
additional information enzyme shows a broad specificity for the aglycone moiety and a strict specificity for the glycone moiety preferring galactose and fucose in the substrate. Enzyme cleaves beta-(1,4) and beta-(1,3) linkages in galacto-oligosaccharides and hydrolyzes the pectic fraction of Arabidopsis cell wall Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
? x * 79000, calculated, x * 75000, SDS-PAGE Arabidopsis thaliana
? x * 81000, calculated, x * 75000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
Gal-2
-
Arabidopsis thaliana
Gal-5
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable up to Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.5
-
Arabidopsis thaliana

pI Value

Organism Comment pI Value Maximum pI Value
Arabidopsis thaliana calculated
-
8.1
Arabidopsis thaliana calculated
-
8.6