Cloned (Comment) | Organism |
---|---|
gene lacL encoding the large subunits of the enzyme, DNA and amino acid sequence determinatin and anaylsis | Lactobacillus acidophilus |
gene lacM encoding the small subunits of the enzyme, DNA and amino acid sequence determinatin and anaylsis | Lactobacillus acidophilus |
General Stability | Organism |
---|---|
Mg2+ is required for enzyme stability | Lactobacillus acidophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-nitrophenyl-beta-D-galactopyranoside | substrate inhibition at high concentrations; substrate inhibition at high concentrations | Lactobacillus acidophilus | |
Ca2+ | 45% inhibition at 10 mM; 45% inhibition at 10 mM | Lactobacillus acidophilus | |
Cu2+ | complete inhibition at 10 mM; complete inhibition at 10 mM | Lactobacillus acidophilus | |
Mn2+ | complete inhibition at 10 mM; complete inhibition at 10 mM | Lactobacillus acidophilus | |
Zn2+ | complete inhibition at 10 mM; complete inhibition at 10 mM | Lactobacillus acidophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.73 | - |
2-nitrophenyl-beta-D-galactopyranoside | pH 6.5, 30°C | Lactobacillus acidophilus | |
4.04 | - |
lactose | pH 6.5, 30°C | Lactobacillus acidophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates 21-28% at 1-10 mM | Lactobacillus acidophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
1 * 35000 + 1 * 72000, SDS-PAGE | Lactobacillus acidophilus |
72000 | - |
1 * 35000 + 1 * 72000, SDS-PAGE | Lactobacillus acidophilus |
105000 | - |
native PAGE | Lactobacillus acidophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus acidophilus | A0SWS3 | large subunit, gene lacL; genes lacL and lacM encoding the two subunits of the enzyme | - |
Lactobacillus acidophilus | A0SWS4 | small subunit, gene lacM; genes lacL and lacM encoding the two subunits of the enzyme | - |
Lactobacillus acidophilus R22 | A0SWS3 | large subunit, gene lacL; genes lacL and lacM encoding the two subunits of the enzyme | - |
Lactobacillus acidophilus R22 | A0SWS4 | small subunit, gene lacM; genes lacL and lacM encoding the two subunits of the enzyme | - |
Purification (Comment) | Organism |
---|---|
native enzyme 28fold to homogeneity by ammonium sulfate fractionation, and hydrophobic interaction and 4-aminobenzyl thiogalactoside affinity chromatography | Lactobacillus acidophilus |
Storage Stability | Organism |
---|---|
22°C, purified enzyme, half-life is 180 h | Lactobacillus acidophilus |
37°C, purified enzyme, half-life is 120 h | Lactobacillus acidophilus |
45°C, purified enzyme, half-life is 24 h | Lactobacillus acidophilus |
4°C, purified enzyme, completely stable for 30 days, 90% remaining activity after 45 days, | Lactobacillus acidophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Lactobacillus acidophilus | 2-nitrophenol + beta-D-galactose | - |
? | |
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Lactobacillus acidophilus R22 | 2-nitrophenol + beta-D-galactose | - |
? | |
lactose + H2O | - |
Lactobacillus acidophilus | D-glucose + D-galactose | - |
? | |
lactose + H2O | - |
Lactobacillus acidophilus R22 | D-glucose + D-galactose | - |
? | |
additional information | the enzyme performs the synthesis of prebiotic galacto-oligosaccharides from lactose with the maximum yield of 38.5% of total sugars at about 75% lactose conversion, overview | Lactobacillus acidophilus | ? | - |
? | |
additional information | the enzyme performs the synthesis of prebiotic galacto-oligosaccharides from lactose with the maximum yield of 38.5% of total sugars at about 75% lactose conversion, overview | Lactobacillus acidophilus R22 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 35000 + 1 * 72000, SDS-PAGE | Lactobacillus acidophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Lactobacillus acidophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 6.8 | - |
Lactobacillus acidophilus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
37.1 | - |
2-nitrophenyl-beta-D-galactopyranoside | pH 6.5, 30°C | Lactobacillus acidophilus |