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Literature summary for 3.2.1.23 extracted from

  • Nguyen, T.H.; Splechtna, B.; Krasteva, S.; Kneifel, W.; Kulbe, K.D.; Divne, C.; Haltrich, D.
    Characterization and molecular cloning of a heterodimeric beta-galactosidase from the probiotic strain Lactobacillus acidophilus R22 (2007), FEMS Microbiol. Lett., 269, 136-144.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene lacL encoding the large subunits of the enzyme, DNA and amino acid sequence determinatin and anaylsis Lactobacillus acidophilus
gene lacM encoding the small subunits of the enzyme, DNA and amino acid sequence determinatin and anaylsis Lactobacillus acidophilus

General Stability

General Stability Organism
Mg2+ is required for enzyme stability Lactobacillus acidophilus

Inhibitors

Inhibitors Comment Organism Structure
2-nitrophenyl-beta-D-galactopyranoside substrate inhibition at high concentrations; substrate inhibition at high concentrations Lactobacillus acidophilus
Ca2+ 45% inhibition at 10 mM; 45% inhibition at 10 mM Lactobacillus acidophilus
Cu2+ complete inhibition at 10 mM; complete inhibition at 10 mM Lactobacillus acidophilus
Mn2+ complete inhibition at 10 mM; complete inhibition at 10 mM Lactobacillus acidophilus
Zn2+ complete inhibition at 10 mM; complete inhibition at 10 mM Lactobacillus acidophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.73
-
2-nitrophenyl-beta-D-galactopyranoside pH 6.5, 30°C Lactobacillus acidophilus
4.04
-
lactose pH 6.5, 30°C Lactobacillus acidophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates 21-28% at 1-10 mM Lactobacillus acidophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
1 * 35000 + 1 * 72000, SDS-PAGE Lactobacillus acidophilus
72000
-
1 * 35000 + 1 * 72000, SDS-PAGE Lactobacillus acidophilus
105000
-
native PAGE Lactobacillus acidophilus

Organism

Organism UniProt Comment Textmining
Lactobacillus acidophilus A0SWS3 large subunit, gene lacL; genes lacL and lacM encoding the two subunits of the enzyme
-
Lactobacillus acidophilus A0SWS4 small subunit, gene lacM; genes lacL and lacM encoding the two subunits of the enzyme
-
Lactobacillus acidophilus R22 A0SWS3 large subunit, gene lacL; genes lacL and lacM encoding the two subunits of the enzyme
-
Lactobacillus acidophilus R22 A0SWS4 small subunit, gene lacM; genes lacL and lacM encoding the two subunits of the enzyme
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 28fold to homogeneity by ammonium sulfate fractionation, and hydrophobic interaction and 4-aminobenzyl thiogalactoside affinity chromatography Lactobacillus acidophilus

Storage Stability

Storage Stability Organism
22°C, purified enzyme, half-life is 180 h Lactobacillus acidophilus
37°C, purified enzyme, half-life is 120 h Lactobacillus acidophilus
45°C, purified enzyme, half-life is 24 h Lactobacillus acidophilus
4°C, purified enzyme, completely stable for 30 days, 90% remaining activity after 45 days, Lactobacillus acidophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
Lactobacillus acidophilus 2-nitrophenol + beta-D-galactose
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
-
Lactobacillus acidophilus R22 2-nitrophenol + beta-D-galactose
-
?
lactose + H2O
-
Lactobacillus acidophilus D-glucose + D-galactose
-
?
lactose + H2O
-
Lactobacillus acidophilus R22 D-glucose + D-galactose
-
?
additional information the enzyme performs the synthesis of prebiotic galacto-oligosaccharides from lactose with the maximum yield of 38.5% of total sugars at about 75% lactose conversion, overview Lactobacillus acidophilus ?
-
?
additional information the enzyme performs the synthesis of prebiotic galacto-oligosaccharides from lactose with the maximum yield of 38.5% of total sugars at about 75% lactose conversion, overview Lactobacillus acidophilus R22 ?
-
?

Subunits

Subunits Comment Organism
dimer 1 * 35000 + 1 * 72000, SDS-PAGE Lactobacillus acidophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Lactobacillus acidophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 6.8
-
Lactobacillus acidophilus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
37.1
-
2-nitrophenyl-beta-D-galactopyranoside pH 6.5, 30°C Lactobacillus acidophilus