Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain JM109 | Thermus sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme immobilization on acid-cleaned, silanized support | Thermus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic modelling of the thermal and pH inactivation of the free and an immobilized form of the enzyme, detailed overview | Thermus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | Thermus sp. | - |
D-glucose + D-galactose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain JM109 | Thermus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Thermus sp. | 2-nitrophenol + beta-D-galactose | - |
? | |
lactose + H2O | - |
Thermus sp. | D-glucose + D-galactose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Thermus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 90 | kinetic modelling of the thermal and pH inactivation of the free and an immobilized form of the enzyme, inactivation mechanism, detailed overview | Thermus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Thermus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 5.5 | kinetic modelling of the thermal and pH inactivation of the free and an immobilized form of the enzyme, inactivation mechanism, detailed overview | Thermus sp. |
9 | 13 | kinetic modelling of the thermal and pH inactivation of the free and an immobilized form of the enzyme, inactivation mechanism, detailed overview | Thermus sp. |