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Literature summary for 3.2.1.23 extracted from
- Purification and characterization of a novel beta-galactosidase from Bacillus sp. MTCC 3088 (2000), J. Ind. Microbiol. Biotechnol., 24, 58-63.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1-2.5 mM | Bacillus sp. (in: Bacteria) |  |
| Cu2+ | 1-2.5 mM | Bacillus sp. (in: Bacteria) | |
| galactose | - |
Bacillus sp. (in: Bacteria) | |
| Hg2+ | 1-2.5 mM | Bacillus sp. (in: Bacteria) | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.18 | - |
lactose | - |
Bacillus sp. (in: Bacteria) | |
| 6.34 | - |
o-nitrophenyl beta-D-galactopyranoside | - |
Bacillus sp. (in: Bacteria) | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | good activator | Bacillus sp. (in: Bacteria) | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 484000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | - |
MTCC 3088 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose + H2O | - |
Bacillus sp. (in: Bacteria) | D-glucose + D-galactose | - |
? | |
| o-nitrophenyl beta-D-galactopyranoside + H2O | the enzyme is specific for beta-D-anomeric linkages | Bacillus sp. (in: Bacteria) | o-nitrophenol + beta-D-galactose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacillus sp. (in: Bacteria) |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | PAGE | - |
6 |
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