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Literature summary for 3.2.1.23 extracted from

  • Uwajima, T.; Yagi, H.; Terada, O.
    Purification, crystallization and some properties of beta-galactosidase from Saccharomyces fragilis (1972), Agric. Biol. Chem., 36, 570-577.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Saccharomyces fragilis

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 0.1 mM Ba(OH)2, 12% inhibition Saccharomyces fragilis
Cu2+ 0.01 mM, 36% inhibition Saccharomyces fragilis
Hg2+ 0.01 4 mM CuSO4, 36% inhibition Saccharomyces fragilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4
-
o-nitrophenyl-beta-D-galactoside
-
Saccharomyces fragilis
21
-
lactose
-
Saccharomyces fragilis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ maximal activation at 0.1 mM Saccharomyces fragilis
K+ activates Saccharomyces fragilis
Mg2+ maximal activation at 1 mM Saccharomyces fragilis
Mn2+ maximal activation at 0.1 mM Saccharomyces fragilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
203000
-
sedimentation equilibrium method Saccharomyces fragilis

Organism

Organism UniProt Comment Textmining
Saccharomyces fragilis
-
-
-
Saccharomyces fragilis KY5463
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces fragilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
90
-
-
Saccharomyces fragilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + H2O
-
Saccharomyces fragilis D-galactose + D-glucose
-
?
lactose + H2O
-
Saccharomyces fragilis KY5463 D-galactose + D-glucose
-
?
o-nitrophenyl-beta-D-galactoside + H2O
-
Saccharomyces fragilis o-nitrophenol + beta-D-galactose
-
?
o-nitrophenyl-beta-D-galactoside + H2O
-
Saccharomyces fragilis KY5463 o-nitrophenol + beta-D-galactose
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
10 min, stable up to Saccharomyces fragilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
hydrolysis of o-nitrophenyl-beta-D-galactoside Saccharomyces fragilis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 pH 5.5: about 30% of maximal activity, pH 7.5: about 50% of maximal activity Saccharomyces fragilis