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Literature summary for 3.2.1.23 extracted from

  • Ikura, Y.; Horikoshi, K.
    beta-Galactosidase in alkalophilic Bacillus (1979), Agric. Biol. Chem., 43, 1359-1360.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag2+ 0.1 mM, complete inactivation Bacillus sp. (in: Bacteria)
Cu2+ 1 mM, 95% inhibition Bacillus sp. (in: Bacteria)
D-galactose 10 mM, 60% loss of activity Bacillus sp. (in: Bacteria)
D-glucose 10 mM, 15% inhibition Bacillus sp. (in: Bacteria)
Fe2+ 1 mM, 38% inhibition Bacillus sp. (in: Bacteria)
Hg2+ 0.1 mM, complete inactivation Bacillus sp. (in: Bacteria)
melibiose 10 mM, 49% inhibition Bacillus sp. (in: Bacteria)
Pb2+ 0.1 mM, complete inactivation Bacillus sp. (in: Bacteria)
xylose no inhibition Bacillus sp. (in: Bacteria)
Zn2+ 1 mM, 90% loss of activity Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9.26
-
o-nitrophenyl-beta-D-galactoside pH 7.6 enzyme Bacillus sp. (in: Bacteria)
9.35
-
o-nitrophenyl-beta-D-galactoside pH 10.2 enzyme Bacillus sp. (in: Bacteria)
48.8
-
lactose pH 10.2 enzyme Bacillus sp. (in: Bacteria)
50.5
-
lactose pH 7.6 enzyme Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
spheroplast 97% of the total activity Bacillus sp. (in: Bacteria)
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
185000
-
gel filtration Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
No. C-125
-
Bacillus sp. (in: Bacteria) No. C-125
-
No. C-125
-

Purification (Commentary)

Purification (Comment) Organism
enzyme from cells grown at pH 7.6 and enzyme from cells grown at pH 10.2 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + H2O
-
Bacillus sp. (in: Bacteria) D-glucose + D-galactose
-
?
lactose + H2O
-
Bacillus sp. (in: Bacteria) No. C-125 D-glucose + D-galactose
-
?
o-nitrophenyl-beta-D-galactoside + H2O
-
Bacillus sp. (in: Bacteria) o-nitrophenol + beta-D-galactose
-
?
o-nitrophenyl-beta-D-galactoside + H2O
-
Bacillus sp. (in: Bacteria) No. C-125 o-nitrophenol + beta-D-galactose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 30°C: about 45% of maximal activity, 55°C: about 25% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
38
-
pH 6.5, 10 min, stable up to Bacillus sp. (in: Bacteria)
55
-
pH 6.5, 10 min, almost all of the activity is lost Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 pH 5.5: about 60% of maximal activity, pH 7.5: about 65% of maximal activity Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9 27°C, 10 min, more than 85% of maximal activity Bacillus sp. (in: Bacteria)