Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag2+ | 0.1 mM, complete inactivation | Bacillus sp. (in: Bacteria) | |
Cu2+ | 1 mM, 95% inhibition | Bacillus sp. (in: Bacteria) | |
D-galactose | 10 mM, 60% loss of activity | Bacillus sp. (in: Bacteria) | |
D-glucose | 10 mM, 15% inhibition | Bacillus sp. (in: Bacteria) | |
Fe2+ | 1 mM, 38% inhibition | Bacillus sp. (in: Bacteria) | |
Hg2+ | 0.1 mM, complete inactivation | Bacillus sp. (in: Bacteria) | |
melibiose | 10 mM, 49% inhibition | Bacillus sp. (in: Bacteria) | |
Pb2+ | 0.1 mM, complete inactivation | Bacillus sp. (in: Bacteria) | |
xylose | no inhibition | Bacillus sp. (in: Bacteria) | |
Zn2+ | 1 mM, 90% loss of activity | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.26 | - |
o-nitrophenyl-beta-D-galactoside | pH 7.6 enzyme | Bacillus sp. (in: Bacteria) | |
9.35 | - |
o-nitrophenyl-beta-D-galactoside | pH 10.2 enzyme | Bacillus sp. (in: Bacteria) | |
48.8 | - |
lactose | pH 10.2 enzyme | Bacillus sp. (in: Bacteria) | |
50.5 | - |
lactose | pH 7.6 enzyme | Bacillus sp. (in: Bacteria) |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
spheroplast | 97% of the total activity | Bacillus sp. (in: Bacteria) | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
185000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
No. C-125 | - |
Bacillus sp. (in: Bacteria) No. C-125 | - |
No. C-125 | - |
Purification (Comment) | Organism |
---|---|
enzyme from cells grown at pH 7.6 and enzyme from cells grown at pH 10.2 | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | - |
Bacillus sp. (in: Bacteria) | D-glucose + D-galactose | - |
? | |
lactose + H2O | - |
Bacillus sp. (in: Bacteria) No. C-125 | D-glucose + D-galactose | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | - |
Bacillus sp. (in: Bacteria) | o-nitrophenol + beta-D-galactose | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | - |
Bacillus sp. (in: Bacteria) No. C-125 | o-nitrophenol + beta-D-galactose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | 30°C: about 45% of maximal activity, 55°C: about 25% of maximal activity | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
pH 6.5, 10 min, stable up to | Bacillus sp. (in: Bacteria) |
55 | - |
pH 6.5, 10 min, almost all of the activity is lost | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | pH 5.5: about 60% of maximal activity, pH 7.5: about 65% of maximal activity | Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | 27°C, 10 min, more than 85% of maximal activity | Bacillus sp. (in: Bacteria) |