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Literature summary for 3.2.1.23 extracted from

  • Kiyohara, T.; Terao, T.; Shioiri-Nakano, K.; Osawa, T.
    Purification and characterization of beta-N-acetylhexosaminidases and beta-galactosidase from Streptococcus 6646 K (1976), J. Biochem., 80, 9-17.
    View publication on PubMed
Search for more literature for 3.2.1.23

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Streptococcus sp.
EDTA 1 mM, 80% loss of activity Streptococcus sp.
Fe2+
-
 Streptococcus sp.
Hg2+
-
 Streptococcus sp.
Pb2+
-
 Streptococcus sp.
Zn2+
-
 Streptococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.17
-
 o-nitrophenyl-beta-D-galactoside
-
 Streptococcus sp.
2.17
-
 p-nitrophenyl-beta-D-galactoside
-
 Streptococcus sp.
4.26
-
 phenyl-beta-D-galactoside
-
 Streptococcus sp.
5.88
-
 lactosyl ceramide
-
 Streptococcus sp.
6.25
-
 lactose
-
 Streptococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Streptococcus sp.
Mg2+ activates Streptococcus sp.
Mn2+ 10 mM MnCl2, 2fold activation Streptococcus sp.

Organism

Organism UniProt Comment Textmining
Streptococcus sp.
-
-
-
Streptococcus sp. 6646 K
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptococcus sp.

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Streptococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + H2O
-
 Streptococcus sp. D-glucose + D-galactose
-
 ?
lactose + H2O
-
 Streptococcus sp. 6646 K D-glucose + D-galactose
-
 ?
lactosyl ceramide + H2O
-
 Streptococcus sp. ?
-
 ?
methyl beta-D-galactohexaoside + H2O
-
 Streptococcus sp. ?
-
 ?
methyl beta-D-galactohexaoside + H2O
-
 Streptococcus sp. 6646 K ?
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Streptococcus sp. o-nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Streptococcus sp. 6646 K o-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-galactoside + H2O
-
 Streptococcus sp. p-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-galactoside + H2O
-
 Streptococcus sp. 6646 K p-nitrophenol + beta-D-galactose
-
 ?
phenyl-beta-D-galactoside + H2O
-
 Streptococcus sp. phenol + beta-D-galactose
-
 ?
phenyl-beta-D-galactoside + H2O
-
 Streptococcus sp. 6646 K phenol + beta-D-galactose
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 5.5, 0.1 M sodium acetate buffer, rapid inactivation above Streptococcus sp.