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Literature summary for 3.2.1.23 extracted from
- Purification and characterization of beta-galactosidase from cell suspension cultures of Marchantia polymorpha (1986), Plant Sci., 44, 97-104.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag2+ | - |
Marchantia polymorpha |  |
| Cu2+ | - |
Marchantia polymorpha | |
| D-galactono-1,4-lactone | - |
Marchantia polymorpha | |
| D-galactose | - |
Marchantia polymorpha | |
| D-glucono-delta-lactone | - |
Marchantia polymorpha | |
| Hg2+ | - |
Marchantia polymorpha | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.625 | - |
p-nitrophenyl beta-D-galactopyranoside | - |
Marchantia polymorpha | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
1 * 62000, SDS-PAGE | Marchantia polymorpha |
| 67000 | - |
gel filtration | Marchantia polymorpha |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Marchantia polymorpha | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Marchantia polymorpha |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Marchantia polymorpha | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Marchantia polymorpha |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least 2 months | Marchantia polymorpha |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| galactan + H2O | - |
Marchantia polymorpha | galactose | + small amounts of glucose and arabinose | ? | |
| p-nitrophenyl-beta-D-galactoside + D-glucose | transgalactosylation | Marchantia polymorpha | lactose + p-nitrophenol | - |
? | |
| p-nitrophenyl-beta-D-galactoside + H2O | - |
Marchantia polymorpha | p-nitrophenol + beta-D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 62000, SDS-PAGE | Marchantia polymorpha |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Marchantia polymorpha |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 4.0, 20 min, 50% loss of activity | Marchantia polymorpha |
| 60 | - |
pH 4.0, 20 min, complete loss of activity | Marchantia polymorpha |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.6 | - |
glycine/HCl buffer | Marchantia polymorpha |
| 3.4 | - |
McIlvaine buffer | Marchantia polymorpha |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.2 | 4.6 | about 50% of maximal activity at pH 2.2 and at pH 4.6 | Marchantia polymorpha |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | 30°C, 16 h, stable | Marchantia polymorpha |
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