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BRENDA support

Literature summary for 3.2.1.23 extracted from

  • Saito, T.; Honda, H.; Iijima, S.; Kobayashi, T.
    Isolation of thermostable beta-galactosidase gene from a thermophilic anaerobe and its expression in Escherichia coli (1989), Enzyme Microb. Technol., 11, 302-305.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacteria

Organism

Organism UniProt Comment Textmining
Bacteria
-
obligatory anaerobic, thermophilic, Gram-gegative strain NA10
-

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
78
-
-
Bacteria

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
3 h, in presence of 2-mercaptoethanol, enzyme from transformant and from strain NA10, stable Bacteria
75
-
3 h, in presence of 2-mercaptoethanol, enzyme from transformant is stable, enzyme form strain NA10 loses 29% of its activity Bacteria
80
-
3 h, 37% loss of activity of transformant enzyme, 95% loss of activity of enzyme form strain NA10 Bacteria

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Bacteria