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Literature summary for 3.2.1.22 extracted from

  • Cobucci-Ponzano, B.; Zorzetti, C.; Strazzulli, A.; Carillo, S.; Bedini, E.; Corsaro, M.; Comfort, D.; Kelly, R.; Rossi, M.; Moracci, M.
    A novel alpha-D-galactosynthase from Thermotoga maritima converts beta-D-galactopyranosyl azide to alpha-galacto-oligosaccharides (2011), Glycobiology, 21, 448-456 .
    View publication on PubMed

Application

Application Comment Organism
synthesis the mutant is an efficient alpha-galactosynthase producing different galactosylated disaccharides from beta-galactosyl-azide donors and 4-nitrophenyl-alpha-and beta-glycosides as acceptors Thermotoga maritima

Protein Variants

Protein Variants Comment Organism
D327G the mutant is an efficient alpha-galactosynthase producing different galactosylated disaccharides from beta-galactosyl-azide donors and 4-nitrophenyl-alpha-and beta-glycosides as acceptors Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 65°C, wild-type enzyme Thermotoga maritima
0.1
-
4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 65°C, mutant enzyme D327G Thermotoga maritima
0.12
-
4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 65°C, mutant enzyme D327G, assay performed in 500 mM NaN3 Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima G4FEF4
-
-
Thermotoga maritima DSM 3109 G4FEF4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-galactopyranoside + H2O
-
Thermotoga maritima 4-nitrophenol + D-galactopyranose
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
-
Thermotoga maritima DSM 3109 4-nitrophenol + D-galactopyranose
-
?

Synonyms

Synonyms Comment Organism
TM1192
-
Thermotoga maritima
TmGalA
-
Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 65°C, mutant enzyme D327G Thermotoga maritima
2.07
-
4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 65°C, mutant enzyme D327G, assay performed in 500 mM NaN3 Thermotoga maritima
65
-
4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 65°C, wild-type enzyme Thermotoga maritima

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.6
-
4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 65°C, mutant enzyme D327G Thermotoga maritima
17
-
4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 65°C, mutant enzyme D327G, assay performed in 500 mM NaN3 Thermotoga maritima
1585
-
4-nitrophenyl alpha-D-galactopyranoside pH 5.0, 65°C, wild-type enzyme Thermotoga maritima