Application | Comment | Organism |
---|---|---|
synthesis | the mutant is an efficient alpha-galactosynthase producing different galactosylated disaccharides from beta-galactosyl-azide donors and 4-nitrophenyl-alpha-and beta-glycosides as acceptors | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
D327G | the mutant is an efficient alpha-galactosynthase producing different galactosylated disaccharides from beta-galactosyl-azide donors and 4-nitrophenyl-alpha-and beta-glycosides as acceptors | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH 5.0, 65°C, wild-type enzyme | Thermotoga maritima | |
0.1 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH 5.0, 65°C, mutant enzyme D327G | Thermotoga maritima | |
0.12 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH 5.0, 65°C, mutant enzyme D327G, assay performed in 500 mM NaN3 | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | G4FEF4 | - |
- |
Thermotoga maritima DSM 3109 | G4FEF4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermotoga maritima | 4-nitrophenol + D-galactopyranose | - |
? | |
4-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermotoga maritima DSM 3109 | 4-nitrophenol + D-galactopyranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TM1192 | - |
Thermotoga maritima |
TmGalA | - |
Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH 5.0, 65°C, mutant enzyme D327G | Thermotoga maritima | |
2.07 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH 5.0, 65°C, mutant enzyme D327G, assay performed in 500 mM NaN3 | Thermotoga maritima | |
65 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH 5.0, 65°C, wild-type enzyme | Thermotoga maritima |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH 5.0, 65°C, mutant enzyme D327G | Thermotoga maritima | |
17 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH 5.0, 65°C, mutant enzyme D327G, assay performed in 500 mM NaN3 | Thermotoga maritima | |
1585 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH 5.0, 65°C, wild-type enzyme | Thermotoga maritima |