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Literature summary for 3.2.1.22 extracted from
- Purification and characterization of a thermostable alpha-galactosidase from Thielavia terrestris NRRL 8126 in solid state fermentation (2012), Acta Biol. Hung., 63, 138-150 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.31 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH 5.0, 37°C | Thermothielavioides terrestris |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 5 mM, 37% residual activity | Thermothielavioides terrestris | |
| Ba2+ | 5 mM, 87% residual activity | Thermothielavioides terrestris | |
| EDTA | 5 mM, 88% residual activity | Thermothielavioides terrestris | |
| Fe2+ | 5 mM, 77% residual activity | Thermothielavioides terrestris | |
| Hg2+ | 5 mM, 24% residual activity | Thermothielavioides terrestris | |
| iodoacetate | 5 mM, 43% residual activity | Thermothielavioides terrestris | |
| L-cysteine | 5 mM, 90% residual activity | Thermothielavioides terrestris | |
| Mg2+ | 5 mM, 90% residual activity | Thermothielavioides terrestris | |
| Mn2+ | 5 mM, 95% residual activity | Thermothielavioides terrestris | |
| Sodium arsenate | 5 mM, 72% residual activity | Thermothielavioides terrestris | |
| Zn2+ | 5 mM, 42% residual activity | Thermothielavioides terrestris | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermothielavioides terrestris | G2RGW4 | - |
- |
| Thermothielavioides terrestris NRRL 8126 | G2RGW4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermothielavioides terrestris |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 85.8 | - |
pH 5.0, 37°C | Thermothielavioides terrestris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermothielavioides terrestris | 4-nitrophenol + alpha-D-galactose | - |
? | |
| 4-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermothielavioides terrestris NRRL 8126 | 4-nitrophenol + alpha-D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 82000, SDS-PAGE | Thermothielavioides terrestris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Thermothielavioides terrestris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
half-life 45 min | Thermothielavioides terrestris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Thermothielavioides terrestris |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Thermothielavioides terrestris | Cicer arietinum (chick pea seed) is the best substrate for alpha-galactosidase production | up |
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